{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:41:43Z","timestamp":1791837703033,"version":"4.3.4"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study investigated the design, synthesis, screening, and functionalization of a novel compound,1,6\u2010diamino\u20104\u2010(3,5\u2010dimethoxyphenyl)\u20102\u2010oxo\u20101,2\u2010dihydropyridine\u20103,5dicarbonitrile (\n                    <jats:bold>4<\/jats:bold>\n                    ), utilizing active carbonyl compounds, acetic acid, and aldehydes to generate new triazolopyridine derivatives. The molecular structures of these derivatives were elucidated through various spectroscopic methods and elemental analysis, including FT\u2010IR,\n                    <jats:sup>1<\/jats:sup>\n                    H NMR,\n                    <jats:sup>13<\/jats:sup>\n                    C NMR, and mass spectrometry. Antimicrobial evaluation by the disc diffusion method was conducted against several microorganisms such as\n                    <jats:italic>Salmonella typhimurium<\/jats:italic>\n                    ,\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ,\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    , and others, with compounds\n                    <jats:bold>4, 5, 6,<\/jats:bold>\n                    and\n                    <jats:bold>10<\/jats:bold>\n                    exhibiting the most significant antimicrobial activity. Furthermore, compounds\n                    <jats:bold>4\u201010<\/jats:bold>\n                    were assessed for them in vitro cytotoxic effects on HepG\u20102, MDA\u2010MB\u2010231, HCT\u2010116, and MCF\u20107 human cancer cell lines, as well as the BJ\u20101 human healthy cell line, using doxorubicin as a reference drug. All tested compounds demonstrated high activity against the hormone\u2010dependent human breast cancer cell line (MCF\u20107) and may serve as promising candidates for this cancer type. Notably, compounds\n                    <jats:bold>4<\/jats:bold>\n                    and\n                    <jats:bold>6<\/jats:bold>\n                    exhibited selective activity against the hormone\u2010dependent human breast cancer (MCF\u20107), human colon cancer (HCT\u2010116), and human liver cancer (HepG2) cell lines, while remaining inactive against the hormone\u2010independent breast cancer cell line (MDA\u2010MB\u2010231).\n                  <\/jats:p>","DOI":"10.1002\/jbt.70743","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T08:40:47Z","timestamp":1770885647000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Novel Triazolopyridine Derivatives: Synthesis, Antimicrobial, Anticancer Evaluation and Molecular Docking Studies"],"prefix":"10.1002","volume":"40","author":[{"given":"Amina A.","family":"Abd El\u2010Gwaad","sequence":"first","affiliation":[{"name":"Department of Therapeutic Chemistry, Pharmaceutical and Drug Industries Research Institute National Research Centre Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina A.","family":"Ali","sequence":"additional","affiliation":[{"name":"Photochemistry Department, Chemical Industries Research Institute National Research Centre Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanem M.","family":"Awad","sequence":"additional","affiliation":[{"name":"Tanning Materials and Leather Technology Department National Research Centre Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman A.","family":"Karam","sequence":"additional","affiliation":[{"name":"Microbial Chemistry Department National Research Centre Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemat S.","family":"Khalaf","sequence":"additional","affiliation":[{"name":"Photochemistry Department, Chemical Industries Research Institute National Research Centre Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2020.112114"},{"key":"e_1_2_7_3_1","first-page":"501","article-title":"Synthesis, Antioxidant Activity and Molecular Docking Study of 1,2,4\u2010Triazole and Their Corresponding Fused Rings Containing 2\u2010Methylphenol","volume":"11","author":"Shakir R. 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