{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T23:57:28Z","timestamp":1777507048951,"version":"3.51.4"},"reference-count":76,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The primary ingredient that gives tobacco its addictive qualities is nicotine (NIC), which constitutes 90%\u201395% of the alkaloids in cigarette smoke. It has been demonstrated that quercetin (Qcn), a significant dietary antioxidant, offers protective benefits in various disease scenarios. Nevertheless, it is unclear if Qcn protects against ovarian toxicity induced by NIC. Therefore, the goal of this work was to examine the potential benefits of Qcn supplementation in reducing NIC\u2010induced toxicity in the caprine ovary in a dose (0.1, 1, and 10\u2009mM) and time (24 and 48\u2009h) dependent manner by performing various biochemical, cytotoxic, and genotoxic studies in vitro, with the proper techniques. As demonstrated by a decrease in apoptotic characteristics or frequency in Qcn + NIC\u2010treated groups compared to the NIC\u2010treated groups alone, Qcn therapy reduced the cytotoxicity in granulosa cells caused by NIC treatment. Treatment with Qcn significantly increases the total antioxidant capacity (higher FRAP activity) and reduces oxidative stress (lower MDA levels) caused by NIC exposure in ovarian tissue, demonstrating Qcn's antioxidant properties. In addition, Qcn supplementation significantly improved the antioxidant status (CAT, SOD, and GST activity) in ovarian tissue after NIC treatment. NIC can harm a variety of biological molecules by potentially causing oxidative stress. According to the study's findings, Qcn treatment has the potential to prevent or at least manage reproductive toxicity in females caused by steadily increasing NIC contamination in the body through various tobacco products, as it significantly reduces NIC\u2010evoked ovarian damage.<\/jats:p>","DOI":"10.1002\/jbt.70758","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T06:44:25Z","timestamp":1771829065000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Protective Effect of Quercetin Against Nicotine\u2010Induced Oxidative Stress and Granulosa Cell Apoptosis in Caprine Ovary In Vitro"],"prefix":"10.1002","volume":"40","author":[{"given":"Anshu","family":"Siwach","sequence":"first","affiliation":[{"name":"Reproductive Physiology Laboratory, Department of Zoology Kurukshetra University Kurukshetra Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2183-3058","authenticated-orcid":false,"given":"Jitender Kumar","family":"Bhardwaj","sequence":"additional","affiliation":[{"name":"Reproductive Physiology Laboratory, Department of Zoology Kurukshetra University Kurukshetra Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,22]]},"reference":[{"key":"e_1_2_13_2_1","unstructured":"\u201cGlobal Burden of Disease \u201d Institute of Health Metrics (IHME) Washington DC 2019."},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgh2015-000005"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.3310\/hta23430"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(95)00037-2"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10254-003-0005-1"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/039463201302600317"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101405"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrb.20052"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101492"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.npp.1300544"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/del487"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-009-9229-4"},{"issue":"1","key":"e_1_2_13_15_1","first-page":"103","article-title":"Garlic and Vitamin E Provides Antioxidant Defence in Tissues of Female Rats Treated With Nicotine","volume":"26","author":"Iranloye B. 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