{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:54:11Z","timestamp":1782860051830,"version":"3.54.5"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:00:00Z","timestamp":1772928000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Short\u2010chain fatty acids (SCFAs), produced through the fermentation of dietary fibers by gut microbiota, play a crucial role in regulating gut microbiota composition, immune function, and overall health. Emerging evidence suggests that SCFAs exhibit antiproliferative effects in various cancers, though the underlying molecular mechanisms remain unclear, particularly in breast cancer (BC). This study aimed to explore the impact of SCFAs, including sodium acetate, propionate, butyrate, and pentanoate, on iron metabolism and ferroptosis in BC cells. The effects of SCFAs were examined in MCF\u20107 and MDA\u2010MB\u2010231 BC cell lines. Cell viability, intracellular iron levels, and expression of iron regulatory proteins transferrin (TfR1), ferroportin (FPN), hepcidin, and ferritin (FTH1) were analyzed. Additionally, oxidative stress markers such as reactive oxygen species (ROS), lipid peroxidation, glutathione peroxidase 4 (GPX4), and mitochondrial membrane potential were assessed. Adenosine triphosphate (ATP) production was also measured to determine ferroptosis involvement. SCFAs exhibited a dose\u2010dependent reduction in BC cell proliferation. They increased intracellular iron content, altered iron metabolism\u2010related protein expression, and elevated oxidative stress. This was evidenced by increased ROS levels, lipid peroxidation, GPX4 downregulation, mitochondrial membrane depolarization, and ATP depletion. SCFAs modulate iron metabolism in BC cells, triggering oxidative stress and ferroptosis\u2010induced cell death. These findings highlight SCFAs' potential as therapeutic agents in BC treatment.<\/jats:p>","DOI":"10.1002\/jbt.70771","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T05:23:12Z","timestamp":1773033792000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Short\u2010Chain Fatty Acids Modulate Iron Metabolism and Induce Oxidative Stress and Ferroptosis in Breast Cancer Cells"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3673-5044","authenticated-orcid":false,"given":"Bilal","family":"Rah","sequence":"first","affiliation":[{"name":"Research Institute of Medical and Health Sciences University of Sharjah Sharjah UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmin","family":"Shafarin","sequence":"additional","affiliation":[{"name":"Research Institute of Medical and Health Sciences University of Sharjah Sharjah UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisha Saleh","family":"Janeeh","sequence":"additional","affiliation":[{"name":"Research Institute of Medical and Health Sciences University of Sharjah Sharjah UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumaisa","family":"Rafiq","sequence":"additional","affiliation":[{"name":"Research Institute of Medical and Health Sciences University of Sharjah Sharjah UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tayma Mohamed Subhi","family":"Kurabi","sequence":"additional","affiliation":[{"name":"Research Institute of Medical and Health Sciences University of Sharjah Sharjah UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9565-1062","authenticated-orcid":false,"given":"Jibran Sualeh","family":"Muhammad","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Medicine and Health University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eyad","family":"Elkord","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioinformatics &amp; 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