{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T20:28:56Z","timestamp":1786566536774,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T00:00:00Z","timestamp":1775433600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Focusing on acute coronary syndrome (ACS), a critical cardiovascular condition, this study explores the diagnostic utility of miR\u20103613\u20103p and its mechanistic involvement in endothelial injury. Quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) quantified serum and cell miR\u20103613\u20103p expression. Receiver operator characteristic (ROC) curve and logistic regression analysis assessed its diagnostic potential. Correlation analysis evaluated the association between miR\u20103613\u20103p and ACS. The effect of miR\u20103613\u20103p on hypoxia\/reoxygenation (H\/R)\u2010induced human coronary artery endothelial cell (HCAEC) injury was evaluated by cell counting kit\u20108 (CCK\u20108, cell proliferation), enzyme linked immunosorbent assay (ELISA) kit (interleukin\u20106, IL\u20106; tumor necrosis factor\u2010\u03b1, TNF\u2010\u03b1), and commercialized assay kits (superoxide dismutase, SOD; glutathione, GSH, malonaldehyde, MDA). Dual\u2010luciferase reporter assay validated the interaction between miR\u20103613\u20103p and ring finger and CCCH\u2010type domains 1 (RC3H1). The recovery experiment assessed the effect of RC3H1 on H\/R\u2010induced HCAEC cell proliferation, inflammation, and oxidative stress. Compared to healthy individuals, serum miR\u20103613\u20103p in ACS, correlating with Gensini score, cardiac troponin I (cTnI), creatine kinase isoenzyme\u2010MB (CK\u2010MB), and left ventricular ejection fraction (LVEF), was downregulated and served as a diagnostic biomarker. In vitro, miR\u20103613\u20103p overexpression promoted cell proliferation, diminished inflammatory factor release, and reduced oxidative stress in H\/R\u2010induced HCAECs. RC3H1 was a direct target of miR\u20103613\u20103p, and its overexpression antagonized the cytoprotective influence of miR\u20103613\u20103p in H\/R\u2010induced HCAEC injury. miR\u20103613\u20103p is a novel biomarker for ACS and provides a new target for ACS intervention by alleviating endothelial damage via targeting RC3H1.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbt.70786","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T03:32:57Z","timestamp":1775532777000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["miR\u20103613\u20103p Is a New Diagnostic Indicator of Acute Coronary Syndrome (ACS) That Reduces Endothelial Damage by Targeting Ring Finger and CCCH\u2010Type Domains 1 (RC3H1)"],"prefix":"10.1002","volume":"40","author":[{"given":"Jianfei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Cardiology, Institute of Cardiovascular 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