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This study investigates the therapeutic potential of pindolol in a murine model of cancer cachexia. In vitro study evaluated the cachexia\u2010inducing potential of B16F10 melanoma cells and the therapeutic effects of pindolol on 3T3\u2010L1 adipocytes. In vivo, cachexia was induced in male C57BL\/6 mice via subcutaneous B16F10 melanoma cell injection, followed by pindolol treatment until the tumor volume reached 5000\u2009mm\u00b3. Body weight, food intake, inflammatory markers, muscle and adipose tissue mass, adipokine levels, metabolic markers, organ weights, and gene expression of E3 ubiquitin\u2010protein ligase TRIM63, Muscle Atrophy F\u2010box gene (atrogin\u20101), and hormone sensitive lipase (HSL) were assessed. B16F10\u2010conditioned media along with isoproterenol induced lipolysis in 3T3\u2010L1 adipocytes, while pindolol reversed this effect. In vivo, pindolol significantly improved body weight, food intake, and systemic inflammation while restoring adipokine levels. Pindolol also preserved skeletal muscle and adipose depots, improved glucose levels, enhanced insulin sensitivity, and lowered triglycerides and cholesterol. Histological analysis confirmed muscle and adipose tissue preservation. Pindolol downregulated TRIM63 and Atrogin\u20101, reducing muscle atrophy, and decreased HSL, suggesting reduced lipolysis evident from mRNA expression. Pindolol alleviates cancer cachexia by reducing inflammation, preserving muscle and adipose mass, and improving metabolic parameters, highlighting its potential as a therapeutic candidate.<\/jats:p>","DOI":"10.1002\/jbt.70812","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T07:20:59Z","timestamp":1774250459000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pindolol Mitigates Cancer Cachexia by Modulating Inflammation, Lipolysis, and Muscle Atrophy"],"prefix":"10.1002","volume":"40","author":[{"given":"Mit","family":"Joshi","sequence":"first","affiliation":[{"name":"Institute of Pharmacy Nirma University Ahmedabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0594-5452","authenticated-orcid":false,"given":"Bhoomika M.","family":"Patel","sequence":"additional","affiliation":[{"name":"National Forensic Sciences University 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