{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T20:28:42Z","timestamp":1786566522460,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Pancreatic cancer (PC), a malignant neoplasm of the gastrointestinal tract, is linked to dismal prognoses and a restricted response to chemotherapy. Long noncoding RNAs (lncRNAs) play critical roles in regulating PC stemness. This study investigated the mechanism of cancer\u2010associated fibroblasts (CAFs)\u2010derived lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) promoting PC cell stemness\n                      <jats:italic>via<\/jats:italic>\n                      the microRNA\u2010101\u20103p (miR\u2010101\u20103p)\/lysine demethylase 3A (KDM3A) axis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Human pancreatic CAFs were cultured in vitro and transfected with small interfering (si)\u2010NEAT1. Extracellular vesicles (EVs) were isolated from CAFs using ultracentrifugation and identified using transmission electron microscopy, nanoparticle tracking analysis, and western blot. Human PC cells PANC\u20101 were treated with miR\u2010101\u20103p mimic, si\u2010KDM3A, and EVs. Expression of NEAT1, miR\u2010101\u20103p, KDM3A, achaete\u2010scute family BHLH transcription factor 2 (ASCL2), leucine\u2010rich repeat\u2010containing G\u2010protein coupled receptor 5 (LGR5), octamer\u2010binding transcription factor 4 (Oct4), and SRY\u2010box transcription factor 2 (SOX2), CD44\n                      <jats:sup>+<\/jats:sup>\n                      CD133\n                      <jats:sup>+<\/jats:sup>\n                      cell count, and cell malignant biological behaviors were assessed. miR\u2010101\u20103p\u2010KDM3A targeted binding sites were predicted by StarBase database, with the targeted relationship verified by the dual\u2010luciferase reporter assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      After CAFs\u2010derived EVs (CAFs\u2010EVs) treatment, PANC\u20101 cells exhibited increased NEAT1, ASCL2, LGR5, Oct4, and SOX2 expression, cell malignant biological behaviors and stemness ability, and CD44\n                      <jats:sup>+<\/jats:sup>\n                      CD133\n                      <jats:sup>+<\/jats:sup>\n                      cell count. CAFs\u2010EVs\u2010delivered NEAT1 potentiated PANC\u20101 cell stemness by downregulating miR\u2010101\u20103p. NEAT1 upregulated KDM3A expression by inhibiting miR\u2010101\u20103p. KDM3A knockdown partly reversed CAFs\u2010EVs\u2010carried NEAT1\u2010promoted PANC\u20101 cell stemness. CAFs\u2010EVs\u2010shuttled NEAT1 encouraged PANC\u20101 cell stemness via the miR\u2010101\u20103p\/KDM3A axis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>CAFs\u2010EVs might carry lncRNA NEAT1 to inhibit miR\u2010101\u20103p expression, thereby regulating KDM3A and eventually facilitating PC cell stemness.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbt.70817","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T08:06:16Z","timestamp":1776153976000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanism of Cancer\u2010Associated Fibroblasts\u2010Derived NEAT1 Promoting Pancreatic Cancer Cell Stemness via the miR\u2010101\u20103p\/KDM3A Axis"],"prefix":"10.1002","volume":"40","author":[{"given":"Jian","family":"Li","sequence":"first","affiliation":[{"name":"Department of General Surgery, Changsha Hospital, Xiangya School of Medicine Central South University Changsha Hunan China"},{"name":"Department of Hepatobiliary Surgery, Changsha Hospital, Xiangya School of Medicine Central South University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Changsha Hospital, Xiangya School of Medicine Central South University Changsha Hunan China"},{"name":"Department of Hepatobiliary Surgery, Changsha Hospital, Xiangya School of Medicine Central South University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6585-4561","authenticated-orcid":false,"given":"Dayong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Changsha Hospital, Xiangya School of Medicine Central South University Changsha Hunan China"},{"name":"Department of Gastrointestinal Surgery Zone 2, Changsha Hospital, Xiangya School of Medicine Central South University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21871"},{"issue":"1","key":"e_1_2_10_3_1","first-page":"19","article-title":"[Report of Cancer Epidemiology in China, 2015]","volume":"41","author":"Zheng R. 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