{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T20:28:53Z","timestamp":1786566533040,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Long\u2010term exposure to the chemical industry pollutant benzene induces hematopoietic damage; however, the mechanism of this injury is unclear. Using an integrative RNA\u2010seq analysis of benzene\u2010exposed samples, we identified Y\u2010box binding protein 1 (YBX1) and neurogenic locus notch homolog protein 1 (NOTCH1) as the most significantly dysregulated apoptosis\u2010related molecules, prompting us to investigate their functional interplay. While neurogenic locus notch homolog protein 1 (NOTCH1) drives the progression of both benign and malignant disorders, the involvement of Y\u2010box binding protein 1 (YBX1) despite being extensively characterized in tumorigenesis remains unexplored in the context of benzene\u2010induced hematopoietic injury. This study examines the molecular mechanism of the YBX1\/NOTCH1 axis in benzene\u2010mediated monocyte apoptosis. Accordingly, we combined clinical data with RNA seqenceing (RNA\u2010seq) analysis and examined the mechanistic basis of benzene\u2010induced apoptosis in human monocytic leukemia cells using Cell Counting Kit\u20108 (CCK\u20108) cell viability assays, flow cytometry (FC), DNA pull\u2010down coupled with liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS), and western blotting (WB). Analysis of 1,4\u2010BQ\u2010treated THP\u20101 cells revealed that 1,4\u2010Benzoquinone (1,4\u2010BQ) upregulated NOTCH1\/transcription coactivator BCL3 (BCL3) and downregulated YBX1\/apoptosis regulator Bcl\u20102 (BCL2) in their gene profiles and protein levels. Furthermore, the differentially expressed genes were enriched in apoptotic pathways. Treatment with 1,4\u2010BQ inhibited THP\u20101 cell proliferation, induced S\u2010phase arrest, and triggered apoptosis in a concentration\u2010 and time\u2010dependent manner. Knockdown of NOTCH1 or overexpression YBX1 significantly eliminated the inhibitory effects of 1,4\u2010BQ on upregulated BCL3 and downregulated BCL2 expression. Furthermore, the inhibitory effects on apoptosis were considerably offset by YBX1 overexpression or NOTCH1 knockdown. YBX1 regulates BCL2\/BCL3 by inhibiting NOTCH1, mediating benzene\u2010induced monocyte apoptosis. Taken together, our results uncovered a critical role of YBX1\/NOTCH1 in the benzene\u2010induced monocyte apoptosis, which may have great potential as a biomarker and therapeutic target in benzene\u2010induced hematotoxicity.<\/jats:p>","DOI":"10.1002\/jbt.70827","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T07:08:07Z","timestamp":1776323287000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanism of YBX1\/NOTCH1 Axis\u2010Mediated Monocyte Apoptosis Under Benzene Exposure"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3458-9859","authenticated-orcid":false,"given":"Qi Jun","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The Affiliated Guangzhou Twelfth People's Hospital Guangzhou Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li 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