{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T05:24:14Z","timestamp":1784957054103,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Long non\u2010coding RNAs (lncRNAs) play crucial roles in hepatocellular carcinoma (HCC), offering valuable insights for therapeutic development. However, the clinical significance and functional mechanisms of LINC00853 in HCC remain poorly understood. This study aimed to evaluate the prognostic value of LINC00853 in HCC patients, and to investigate its underlying mechanisms in regulating HCC. A cohort of 123 HCC patients was enrolled in this study. LINC00853 expression in tumor tissues was quantified by quantitative reverse transcription\u2010PCR (qRT\u2010PCR). Prognostic significance of LINC00853 was assessed using Kaplan\u2013Meier curves and Cox regression models. In vitro functional assays, including Cell Counting Kit\u20108 (CCK\u20108) and Transwell assays, were conducted to evaluate the effects of LINC00853 knockdown on cell proliferation, migration, and invasion. The regulatory axis involving LINC00853, miR\u201016\u20105p, and programmed death\u2010ligand 1 (PD\u2010L1) was validated by dual\u2010luciferase reporter assays. The role of LINC00853 in immune escape was investigated by co\u2010culturing CD8+ T cells with HCC cells and measuring cytotoxic activity along with cytokine (TNF\u2010\u03b1, IFN\u2010\u03b3, IL\u201010, and TGF\u2010\u03b2) levels. LINC00853 was significantly overexpressed in HCC tissues and was associated with unfavorable prognosis. Silencing LINC00853 suppressed the proliferation, migration, and invasion of HCC cells. Mechanistically, LINC00853 functioned as a molecular sponge for miR\u201016\u20105p, thereby upregulating PD\u2010L1 expression. In the co\u2010culture system, LINC00853 knockdown enhanced CD8+ T cell cytotoxicity and promoted the secretion of inflammatory cytokines (TNF\u2010\u03b1 and IFN\u2010\u03b3), while reducing immunosuppressive factors (IL\u201010 and TGF\u2010\u03b2). These effects were reversed by the miR\u201016\u20105p inhibitor. LINC00853 may serve as a novel prognostic marker for HCC. Knockdown of LINC00853 suppresses the progression and immune escape by targeting the miR\u201016\u20105p\/PD\u2010L1 axis, suggesting its potential as a therapeutic target.<\/jats:p>","DOI":"10.1002\/jbt.70831","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T06:14:07Z","timestamp":1776924847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Knockdown of LINC00853 Inhibits the Progression and Immune Escape of Hepatocellular Carcinoma by Targeting the miR\u201016\u20105p\/PD\u2010L1 Axis"],"prefix":"10.1002","volume":"40","author":[{"given":"Dong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of Wannan Medical University (Yijishan Hospital of Wannan Medical University) Wuhu Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Shandong First Medical University Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of Wannan Medical University (Yijishan Hospital of Wannan Medical University) Wuhu Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0392-8256","authenticated-orcid":false,"given":"Shaocong","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Oncology The First People's Hospital of Jingdezhen Jingdezhen Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2022.08.021"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2022.05.003"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2022.01.023"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-023-00818-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-020-01188-4"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.07.014"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncrna.2024.03.011"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24097923"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/2211-5463.12983"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-80926-w"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12745"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41065-025-00520-6"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.2147\/ijn.S503954"},{"issue":"2","key":"e_1_2_9_15_1","first-page":"175","article-title":"[Prognostic Potential of hsa\u2010miR\u201016\u20105p, hsa\u2010miR\u2010125b\u20105p, and hsa\u2010miR\u2010181a\u20105p for the Formation of Groups of Increased Risk of Breast Cancer Under Radiation Exposure]","volume":"59","author":"Yanishevskaya M. 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