{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:03:42Z","timestamp":1790694222069,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aims to investigate the effects of subchronic exposure to Tebuconazole (TEB) on the parotid salivary gland of adolescent rats. These animals were exposed daily to TEB (10, 20, and 50\u2009mg\/kg, orally) or vehicle for 30 days. The parotid glands were evaluated by histopathological (hematoxylin\u2010eosin staining and histomorphometry), histochemical (Periodic Acid\u2010Schiff [PAS] for glycoconjugates and Picrosirius Red for collagen fibers) and immunohistochemical analysis for markers of oxidative stress (8\u2010OHdG), inflammation (COX\u20102), apoptosis (caspase\u20103), epithelial remodeling (CK7), and cell proliferation (Ki\u201067). Histopathological results showed dose\u2010dependent morphological changes, including architectural disorganization of the glandular parenchyma, cytoplasmic vacuolation, and inflammatory infiltrate. PAS results showed a significant reduction in labeling in the 50\u2010 and 20\u2010mg\/kg groups in relation to the 10\u2009mg\/kg and control groups, evincing a reduction in cell activity. The immunostaining of 8\u2010OHdG (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05 at the 50\u2009mg\/kg dose), caspase\u20103 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05 at all doses), CK7 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05 at the 20\u2010 and 50\u2010mg\/kg doses), and COX\u20102 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05 at all doses) significantly increased. The expression of the proliferation marker Ki\u201067 showed no significant alteration, whereas the histomorphometric analysis confirmed a progressive and significant reduction in cell density (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Moreover, the Picrosirius Red staining technique showed an increase in fibrotic tissue within the glandular parenchyma. In conclusion, this study found that the subchronic exposure to TEB induces dose\u2010dependent toxicity in the parotid gland of adolescent rats.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70833","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T09:53:51Z","timestamp":1775814831000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Tebuconazole Toxicity in the Parotid Gland of Adolescent Rats"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-5951-4399","authenticated-orcid":false,"given":"Gabriel Carvalhal","family":"de Aguiar","sequence":"first","affiliation":[{"name":"Department of Biosciences, Institute of Health and Society, UNIFESP Federal University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2386-2126","authenticated-orcid":false,"given":"Lorrany da Silva","family":"Avanci","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Institute of Health and Society, UNIFESP Federal University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0999-5847","authenticated-orcid":false,"given":"Daniel Vitor","family":"de Souza","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Institute of Health and Society, UNIFESP Federal University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5880-1941","authenticated-orcid":false,"given":"Lucas Vilas\u2010B\u00f4as","family":"Correia","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Institute of Health and Society, UNIFESP Federal University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina Cl\u00e1udia Barbosa da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Institute of Health and Society, UNIFESP Federal University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4408-1503","authenticated-orcid":false,"given":"Flavia","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Institute of Health and Society, UNIFESP Federal University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea Cristina de Moraes","family":"Malinverni","sequence":"additional","affiliation":[{"name":"Department of Pathology, Paulista Medical School, UNIFESP Federal University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Augusto Cintra","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Institute of Health and Society, UNIFESP Federal University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5057-4983","authenticated-orcid":false,"given":"Daniel Araki","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Institute of Health and Society, UNIFESP Federal University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.5772\/13109"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2015.09.008"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/62.2.339"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-018-2587-9"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.5812\/hepatmon.94548"},{"key":"e_1_2_11_7_1","volume-title":"Program for the Analysis of Pesticide Residues in Food\u2014PARA: Report of the Samples Analyzed in the Period 2017\u20132018","author":"ANVISA","year":"2019"},{"key":"e_1_2_11_8_1","volume-title":"Re\u2010evaluation Decision RVD2024\u201009, Tebuconazole and Its Associated End\u2010Use Products","author":"Health Canada","year":"2024"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2012.03.205"},{"key":"e_1_2_11_10_1","volume-title":"Joint FAO Meeting on Pesticide Residues. 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