{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T20:28:42Z","timestamp":1786566522601,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Although natural flavonoids have shown potential in acute kidney injury (AKI) treatment, evidence regarding the renal protective effects of Vitisin A remains limited. We aimed to investigate the protective effects of Vitisin A on renal function and its underlying molecular mechanisms in lipopolysaccharide (LPS)\u2010induced AKI. Renal function was assessed by measuring serum creatinine (Scr), blood urea nitrogen (BUN), and uric acid (UA) in LPS\u2010induced AKI animal models. The expression of renal tubular injury markers (e.g., KIM\u20101, NGAL) was analyzed using qPCR and Western blot (WB) analysis. Oxidative stress, inflammation, and apoptosis were evaluated by detecting pro\u2010oxidant and antioxidant proteins (e.g., NOX4, MDA, GSH), inflammatory cytokines (e.g., TNF\u2010\u03b1, IL\u20106), and apoptotic proteins (e.g., Bcl\u20102, Bax). We also examined the activation of NF\u2010\u03baB and MAPK signaling pathways in LPS group and LPS+ Vitisin A group. LPS treatment impaired renal function, increased the expression of renal tubular injury markers, and induced severe oxidative stress and inflammation in renal tissues. Vitisin A treatment markedly improved renal function, alleviated tubular injury, and reduced the elevated expression of NOX4, and MDA while restoring antioxidant protein levels. Furthermore, Vitisin A attenuated renal inflammation and apoptosis. Mechanistically, Vitisin A inhibited the activation of NF\u2010\u03baB and MAPK pathways, as evidenced by reduced phosphorylation of p65, I\u03baB\u03b1, ERK, JNK, and p38 in LPS\u2010induced AKI mice. Our study demonstrated that Vitisin A improved renal function, reduced tubular injury, and attenuated inflammation, oxidative stress, and apoptosis in LPS\u2010induced AKI mice, accompanied by suppression of MAPK and NF\u2010\u03baB signaling. Our findings provided experimental evidence supporting Vitisin A as a potential therapeutic agent for AKI. However, further research is needed to evaluate its long\u2010term efficacy and safety for clinical translation.<\/jats:p>","DOI":"10.1002\/jbt.70835","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T07:06:54Z","timestamp":1776150414000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vitisin A Inhibits MAPK and NF\u2010\u03baB Signaling Pathways and Improves Renal Function in LPS\u2010Induced Acute Kidney Injury Mice"],"prefix":"10.1002","volume":"40","author":[{"given":"Guangling","family":"Li","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Affiliated Hospital of Jiangnan University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Affiliated Hospital of Jiangnan University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Affiliated Hospital of Jiangnan University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiran","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, The Second School of Clinical Medicine Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2629-9001","authenticated-orcid":false,"given":"Jing","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology the First Affiliated Hospital of Bengbu Medical University Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.113600"},{"issue":"2","key":"e_1_2_10_3_1","first-page":"249","article-title":"Aromadendrin Alleviates LPS\u2010Induced Kidney Apoptosis and Inflammation by Inhibiting Phosphorylation of MAPK and NF\u2010\u03baB Signaling Pathways","volume":"40","author":"Ma X.","year":"2025","journal-title":"Histology and Histopathology"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/CNQ.0000000000000553"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.70064"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2024.10.034"},{"key":"e_1_2_10_7_1","first-page":"103","article-title":"Application of Early Risk Factor Warning Model of Acute Kidney Injury Combined With Continuous Renal Replacement Therapy in Patients With Severe Acute Pancreatitis","volume":"356","author":"Ru L. 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