{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T06:35:54Z","timestamp":1776666954223,"version":"3.51.2"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T00:00:00Z","timestamp":1776556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T00:00:00Z","timestamp":1776556800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      This systematic review aimed to evaluate the cytotoxic effects of green\u2010synthesized silver nanoparticles (GS\u2010AgNPs) on various oral cell lines based on the available evidence. This review was registered on PROSPERO (CRD420251183806) and conducted in accordance with PRISMA 2020 guidelines. A systematic search across four major databases (1990\u20132025) for English\u2010language studies identified in vitro studies assessing GS\u2010AgNPs\u2010induced cytotoxicity in oral cell lines, using assays such as MTT and Annexin V\u2010FITC. The quality of the included studies was evaluated using the QUIN tool for in vitro studies, and the meta\u2010analysis compared cytotoxic responses between normal and cancerous oral cell lines. A total eleven studies met the inclusion criteria, predominantly using the MTT assay to assess cytotoxic responses induced by GS\u2010AgNPs. Two comparative studies (100%) included in the review reported lower toxicity for GS\u2010AgNPs than for conventionally synthesized AgNPs. Cytotoxicity varied with synthesis components, nanoparticle characteristics, concentration, and cell types. GS\u2010AgNPs demonstrated selective cytotoxicity towards oral cancer cell lines, and the meta\u2010analysis confirmed significantly higher cancer\u2010cell sensitivity (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.00001, I\u00b2\u2009=\u20097%). Risk\u2010of\u2010bias assessment indicated overall low to moderate bias. In conclusion, green\u2010synthesized silver nanoparticles demonstrated lower cytotoxicity compared with chemically synthesized silver nanoparticles. The GS\u2010AgNPs also exhibited selective cytotoxicity towards oral cancer cell lines, influenced by nanoparticle size, shape, and concentration. Their favorable biocompatibility and selective anticancer activity support their potential application in oral cancer therapy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbt.70836","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T05:35:55Z","timestamp":1776663355000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cytotoxicity of Green Synthesized Silver Nanoparticles Against Oral Cell Lines: A Systematic Review and Meta\u2010Analysis"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0612-0014","authenticated-orcid":false,"given":"Yogesh J.","family":"Kale","sequence":"first","affiliation":[{"name":"Department of Pediatric and Preventive Dentistry MIDSR Dental College Latur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5476-0931","authenticated-orcid":false,"given":"Shraddha N.","family":"Kasture","sequence":"additional","affiliation":[{"name":"Department of Pediatric and Preventive Dentistry MIDSR Dental College Latur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4315-4432","authenticated-orcid":false,"given":"Mahesh V.","family":"Dadpe","sequence":"additional","affiliation":[{"name":"Department of Pediatric and Preventive Dentistry MIDSR Dental College Latur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0295-5751","authenticated-orcid":false,"given":"Prasanna T.","family":"Dahake","sequence":"additional","affiliation":[{"name":"Department of Pediatric and Preventive Dentistry MIDSR Dental College Latur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3070-0238","authenticated-orcid":false,"given":"Shrikant B.","family":"Kendre","sequence":"additional","affiliation":[{"name":"Department of Pediatric and Preventive Dentistry MIDSR Dental College Latur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb11040084"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.1385"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering10030327"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/coatings12091338"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano12040673"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crgsc.2021.100205"},{"issue":"1","key":"e_1_2_11_8_1","first-page":"210","article-title":"Does Biosynthetic Silver Nanoparticles Are More Stable With Lower Toxicity Than Their Synthetic Counterparts? 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