{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T06:47:45Z","timestamp":1782110865415,"version":"3.54.5"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Endometritis is a prevalent reproductive disorder predominantly triggered by microbial infection. Casticin exhibits diverse pharmacological properties, including anti\u2010inflammatory and anti\u2010tumor activities. However, its protective potential against endometritis remains poorly documented. Thus, this study aimed to investigate the protective efficacy and underlying mechanism of casticin in S. aureus\u2010induced endometritis. ELISA was used to quantify the levels of TNF\u2010\u03b1 and IL\u20101\u03b2, while ferroptosis\u2010related indicators were measured using commercial assay kits and Western blot analysis. The results showed that casticin significantly alleviated\n                    <jats:italic>S. aureus<\/jats:italic>\n                    \u2010induced myeloperoxidase (MPO) activity, TNF\u2010\u03b1 and IL\u20101\u03b2 production, and nuclear factor\u2010kappa B (NF\u2010\u03baB) activation. Additionally, casticin diminished the ferroptosis\u2010associated elevation of malondialdehyde (MDA) levels and intracellular iron content, while concurrently enhancing glutathione (GSH) biosynthesis and upregulating the expression of GPX4 and xCT. Furthermore, the expression of sirtuin 1 (SIRT1), AMP\u2010activated protein kinase (AMPK), and peroxisome proliferator\u2010activated receptor gamma coactivator 1\u2010alpha (PGC\u20101\u03b1) were increased by casticin. In vitro, the inhibition of casticin on\n                    <jats:italic>S. aureus<\/jats:italic>\n                    \u2010induced inflammation and ferroptosis were prevented by AMPK inhibitor compound C or SIRT1 inhibitor EX\u2010527. In conclusion, our findings indicate that casticin mitigates S. aureus\u2010induced endometritis by suppressing inflammation and ferroptosis, a mechanism mediated through the regulation of the AMPK\/SIRT1\/PGC\u20101\u03b1 signaling pathway.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70857","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T12:56:53Z","timestamp":1779713813000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Activation of AMPK\/SIRT1\/PGC\u20101\u03b1 Signaling Pathway by Casticin Protects Against\n                    <i>S. aureus<\/i>\n                    \u2010Induced Endometritis"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8033-1402","authenticated-orcid":false,"given":"Nanzhu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Gynaecology Gynecological Diagnosis and Treatment Center, Affliated Hospital of Changchun University of Chinese Medicine Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gynaecology Gynecological Diagnosis and Treatment Center, Affliated Hospital of Changchun University of Chinese Medicine Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics Gynecological Diagnosis and Treatment Center, Affliated Hospital of Changchun University of Chinese Medicine Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changhui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gynaecology Gynecological Diagnosis and Treatment Center, Affliated Hospital of Changchun University of Chinese Medicine Changchun Jilin 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