{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T14:32:52Z","timestamp":1777300372369,"version":"3.51.4"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Oral Submucous Fibrosis (OSMF) is a chronic, progressive, and potentially malignant condition of the oral cavity. Despite the availability of various therapeutic approaches, no single medication has demonstrated complete efficacy in reversing fibrosis or stopping the disease progression. Sesamol is a naturally occurring phenolic compound that is derived from sesame seeds and has strong anti\u2010inflammatory, anticancer, and antioxidant effects. However, its anti\u2010fibrotic and anti\u2010proliferative properties in OSMF remain underexplored. Therefore, this study aimed to evaluate the anti\u2010proliferative and anti\u2010fibrotic effects of sesamol on the primary OSMF cell line.<\/jats:p>\n                  <jats:p>\n                    This was an In\u2010Vitro Experimental study. The study was conducted in Ziauddin University, Clifton Campus, Karachi, from December 2024 to August 2025. The Primary OSMF fibroblasts (passages p8\u2010p10) were treated with sesamol at its IC\n                    <jats:sub>50<\/jats:sub>\n                    concentration, and the standard drug was dexamethasone (IC\n                    <jats:sub>50<\/jats:sub>\n                    ). Cell viability was assessed using the MTT assay, and the anti\u2010migration ability was examined through the scratch assay. Antifibrotic actions were also evaluated in Masson's trichrome staining (MTS), and quantification of fibrosis\u2010related genes was performed to assess the results.\n                  <\/jats:p>\n                  <jats:p>Sesamol treatment suppressed OSMF cell viability in a dose\u2010dependent manner, demonstrating cytotoxic and significant anti\u2010proliferative effects. Scratch assay analysis showed sesamol demonstrated significant anti\u2010migratory efficacy with delayed wound closure, whereas dexamethasone exerted an inhibitory effect. MTS showed that sesamol markedly reduced collagen buildup, highlighting its anti\u2010fibrotic potential. Gene expression analysis showed that sesamol increased MMP\u20101 and MMP\u20102 while reducing COL1A2, \u03b1\u2010SMA, and TGF\u2010\u03b21, promoting ECM degradation and suppression of fibrotic signaling. In contrast, dexamethasone modulated MMP expression and fibrosis\u2010related genes. Dexamethasone, a standard clinically used corticosteroid for OSMF management, was used as the reference treatment to benchmark the molecular effects of sesamol.<\/jats:p>\n                  <jats:p>In conclusion, sesamol demonstrated a significant anti\u2010proliferative, anti\u2010migratory, and anti\u2010fibrotic effect on primary OSMF fibroblasts, suggesting its potential to influence key profibrotic pathways. These findings highlight sesamol as a promising candidate for further preclinical studies in OSMF management.<\/jats:p>","DOI":"10.1002\/jbt.70863","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T13:33:16Z","timestamp":1777296796000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sesamol As a Potential Anti\u2010Fibrotic Agent Targeting ECM Dynamics in Oral Submucous Fibrosis"],"prefix":"10.1002","volume":"40","author":[{"given":"Samreen","family":"Ramzan","sequence":"first","affiliation":[{"name":"College of Molecular Medicine, Ziauddin University Karachi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amreen","family":"Liaquat","sequence":"additional","affiliation":[{"name":"Ziauddin Medical College, Ziauddin University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2879-4036","authenticated-orcid":false,"given":"Anum","family":"Islam","sequence":"additional","affiliation":[{"name":"Ziauddin College of Dentistry, Ziauddin University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahrukh","family":"Khan","sequence":"additional","affiliation":[{"name":"Ziauddin College of Dentistry, Ziauddin University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6264-3966","authenticated-orcid":false,"given":"Talat","family":"Mirza","sequence":"additional","affiliation":[{"name":"College of Molecular Medicine, Ziauddin University Karachi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0506-5981","authenticated-orcid":false,"given":"Shumaila","family":"Usman","sequence":"additional","affiliation":[{"name":"College of Molecular Medicine, Ziauddin University Karachi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,27]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.53350\/pjmhs2115113058"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/jpo.jpo_23_22"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20122940"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40463-020-0399-7"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/jpbs.jpbs_467_22"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2012.13.1.289"},{"issue":"11","key":"e_1_2_12_8_1","first-page":"1362","article-title":"High Risk of Malignant Transformation of Oral Submucous Fibrosis in Pakistani Females: A Potential National Disaster","volume":"66","author":"Mohiuddin S.","year":"2016","journal-title":"JPMA. 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