{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T06:12:35Z","timestamp":1777961555579,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Chronic stress resulted in poor prognosis of cancer patients in the clinic. This study aimed to explore the mechanism by which chronic stress promotes the progression of lung adenocarcinoma (LUAD) through circ_RPPH1\/miR\u2010326 axis. Chronic stress\u2010induced LUAD in vitro and in vivo models were established in A549 cells and C57BL\/6 male mice. Sucrose preference test (SPT) and forced\u2010swimming test (FST) were used for in vivo model evaluation. qRT\u2010PCR and western blot were used to detect mRNA and protein levels. Cell proliferation, migration and invasion were also evaluated. Luciferase reporter assay was for target gene verification. In A549 cells, acetylcholine (ACh)\u2010induced chronic stress contributed to the upregulation of circ_RPPH1. circ_RPPH1 knockdown remitted the carcinogenic effect of chronic stress on A549 cells. circ_RPPH1 serves as a sponger of miR\u2010326, and miR\u2010326 downregulation neutralized the beneficial role against tumor cell function and EMT in vitro. In vivo, circ_RPPH1 knockdown reduced the tumor volume and EMT\u2010related protein levels of tumor\u2010bearing mice under chronic stress treatment, while miR\u2010326 antagomir co\u2010transfection neutralized the effect. LARP1 might be the target gene of circ_RPPH1\/miR\u2010326 axis in CUMS mice. circ_RPPH1\/miR\u2010326 axis was involved in chronic stress\u2010promoted progression and metastasis of LUAD.<\/jats:p>","DOI":"10.1002\/jbt.70870","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T05:47:04Z","timestamp":1777960024000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Circ_RPPH1\/miR\u2010326 Axis Blocks Chronic Stress\u2010Promoted Progression and Metastasis of Lung Adenocarcinoma"],"prefix":"10.1002","volume":"40","author":[{"given":"Ying","family":"Xiang","sequence":"first","affiliation":[{"name":"Department of Urology School of Medicine UESTC, Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangfeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Proctology School of Medicine UESTC, Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Oncology Center School of Medicine UESTC, Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Jin","sequence":"additional","affiliation":[{"name":"Oncology Center School of Medicine UESTC, Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianmei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Oncology Center School of Medicine UESTC, Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6154-1177","authenticated-orcid":false,"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Oncology Center School of Medicine UESTC, Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,5,4]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ccm.2019.10.001","article-title":"Lung Cancer 2020","volume":"41","author":"Bade B. 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Medical science edition"},{"issue":"1","key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.2174\/1566523221666211202095258","article-title":"CircRNAs in Lung Adenocarcinoma: Diagnosis and Therapy","volume":"22","author":"Su L.","year":"2021","journal-title":"Current Gene Therapy"},{"issue":"1","key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/s41419-020-2230-9","article-title":"circRNA\u2010002178 Act as a ceRNA to Promote PDL1\/PD1 Expression in Lung Adenocarcinoma","volume":"11","author":"Wang J.","year":"2020","journal-title":"Cell Death & Disease"},{"issue":"16","key":"e_1_2_9_9_1","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1111\/1759-7714.14889","article-title":"CircRNA ZKSCAN1 Promotes Lung Adenocarcinoma Progression by miR\u2010185\u20105p\/TAGLN2 Axis","volume":"14","author":"Yu N.","year":"2023","journal-title":"Thoracic 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