{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T16:11:50Z","timestamp":1783699910739,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ulcerative colitis (UC) is a chronic, relapsing disease, imposing a heavy disease burden and severely reducing the quality life of patients. This study verified the influence of the PVT1\/miR\u2010216a\u20105p\/TLR4 signaling axis on UC. LPS\u2010stimulated RAW264.7 cells were co\u2010cultured with Caco\u20102 cells to establish an in vitro model. The C57BL\/6\u2009J mice induced by 3% DSS were used as an in vivo model. Cytotoxicity and barrier function\u00a0were evaluated in Caco\u20102 cells. The PVT1, miR\u2010216a\u20105p, and TLR4 levels were detected by RT\u2010qPCR. ELISA was used to detect the level of pro\u2010inflammatory factors in RAW264.7 supernatant and colon tissue, and the level of ZO\u20101 and occludin in Caco\u20102 cells and colon tissue. TEER was used to evaluate the integrity of the barrier function of Caco\u20102 cells. The targeting relationships between PVT1, miR\u2010216a\u20105p, and TLR4 were verified by dual\u2010luciferase reporter gene assay. The PVT1 and TLR4 levels were increased, but miR\u2010216a\u20105p was decreased in the LPS\u2010treated RAW264.7, co\u2010cultured Caco\u20102, and colon tissue of DSS mice. The pro\u2010inflammatory factor levels in RAW264.7 and colon tissue of DSS mice were increased, while in Caco\u20102, the cytotoxicity was aggravated, and the integrity of the barrier function was disrupted. The levels of ZO\u20101 and occludin were decreased in Caco\u20102 cells and tissue. If the PVT1 expression was inhibited, the results showed an opposite trend. In contrast, inhibition of miR\u2011216a\u20115p reversed the protective effects induced by PVT1 knockdown. The PVT1\/miR\u2010216a\u20105p signaling axis affects the inflammation and intestinal epithelial cell barrier function in UC, which may be a possible therapeutic target for UC.<\/jats:p>","DOI":"10.1002\/jbt.70887","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T09:21:45Z","timestamp":1779096105000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The PVT1\/miR\u2010216a\u20105p\/TLR4 Signaling Axis Regulates the Inflammation and Intestinal Epithelial Barrier Function of Ulcerative Colitis"],"prefix":"10.1002","volume":"40","author":[{"given":"Meixiao","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Basic Medicine Cangzhou Medical College Cangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Cangzhou Medical 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