{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T06:47:50Z","timestamp":1782110870396,"version":"3.54.5"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>ALOX15 exerts tumor suppressor in numerous cancers. However, the role of ALOX15 in lung cancer is scarcely reported. This study aimed to investigate the role of ALOX15 in lung cancer. GSE225620 was used to analyzed the differentially expressed genes in lung cancer patients treated with 5\u2010FU. Gene expression was determined using RT\u2010qPCR, Western blot, and immunofluorescence. Ferroptosis markers were analyzed using ELISA assays. Cytokine release was determined using flow cytometry. Cellular functions were analyzed using CCK\u20108, transwell assay, PI staining, and flow cytometry assays. In vivo assays were conducted to further verify the role of ALOX15 and its metabolite in lung cancer. We found that ALOX15 expression was upregulated in lung cancer patients after 5\u2010FU treatment. ALOX15 mediated the release of 15\u2010HETE, driving lung cancer cells to ferroptosis. Moreover, ALOX15\u2010mediated accumulation of 15\u2010HETE stimulated anti\u2010tumor CD8\u2009+\u2009T cell immunity in vivo and in vitro. Mechanistically, ALOX15 upregulated TCF1, promoting the stem\u2010like behaviors of CD8\u2009+\u2009T cells. Moreover, upregulated TCF1 suppressed the expression of immune checkpoint genes. ALOX15 functions as an anti\u2010tumor gene in lung cancer. TCF1\u2010mediated 15\u2010HETE uptake drives lung cancer cell ferroptosis and long\u2010existing of CD8\u2009+\u2009T cells. Therefore, ALOX15 may be a potential target for lung cancer.<\/jats:p>","DOI":"10.1002\/jbt.70902","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T05:47:45Z","timestamp":1780465665000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["ALOX15\u2010Mediated 15\u2010HETE Uptake Drives Lung Cancer Cells Ferroptosis and Enhances CD8+ T Cell Immunity in Lung Cancer"],"prefix":"10.1002","volume":"40","author":[{"given":"Jianglong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Thoracic, Cardiac, Breast and Thyroid Surgery Wushan County People's Hospital Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gangyi","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Thoracic, Cardiac, Breast and Thyroid Surgery Wushan County People's Hospital Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4604-1389","authenticated-orcid":false,"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Thoracic, Cardiac, Breast and Thyroid Surgery Wushan County People's Hospital Chongqing 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