{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T06:47:40Z","timestamp":1782110860484,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study was carried out to evaluate the therapeutic efficacy of Neocuproine\/Trolox treatment on chronic neuropathy induced by Chronic Constriction Injury (CCI). Rats (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200940) were grouped as Sham, CCI, CCI+Trolox, and CCI+Neocuproine. Rats with proven neuropathy were given 80\u2009mg\/kg\/day (i.p.) Trolox and 100\u2009\u03bcM\/day (i.p.) Neocuproine for 7 days. Open field and tail withdrawal tests were conducted at baseline and on days 7 and 14. ELISA test was employed in analyzing IL 1\u03b2, IL 10, TNF\u2010\u03b1, and TGF\u2010\u03b2 levels in spinal cord and cortex tissues of rats. To explore potential mechanistic interactions, molecular docking was conducted with the key cytokine receptor. On day 14, a significant decrease in total path taken, number of frames entered, and delay values were observed in CCI group compared to the control, whereas significant increases were observed in total path taken, number of frames entered, and delay values in the CCI+Neocuproine\/Trolox treatment groups compared to CCI group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05 for all). Docking analyses were performed for Neocuproine\/Trolox. In comparison to the sham group, IL\u20111\u03b2 and TNF\u2010\u03b1 levels in spinal cord and cortex tissues were higher, while IL\u201110 and TGF\u2010\u03b2 levels were lower (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Neocuproine\/Trolox treatments reduced proinflammatory cytokine levels and elevated anti\u2010inflammatory cytokine levels in CCI rats on day 14 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05 for all). Considering the docking score of Neocuproine\/Trolox, high scores were observed, especially with TNF\u2010\u03b1 and TGF\u2010\u03b2 receptor. The results suggest that Neocuproine\/Trolox treatments induce thermal anti\u2010hyperalgesic behaviors in CCI\u2010induced pain, showing significant improvements in locomotor activity, and achieve these by reducing the production of proinflammatory cytokines and elevating that of anti\u2010inflammatory cytokines.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70922","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T07:18:32Z","timestamp":1781594312000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Docking and Experimental Evaluation of Trolox and Neocuproine in Targeting Neuroinflammation in Neuropathic Pain Model"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9301-9119","authenticated-orcid":false,"given":"Selma","family":"Yaman","sequence":"first","affiliation":[{"name":"Department of Biophysics Faculty of Medicine 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