{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T06:47:45Z","timestamp":1782110865433,"version":"3.54.5"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Diphtheria toxin (DT) has been reported to exhibit cytotoxic effects in several cancer models; however, the molecular mechanisms underlying its apoptotic activity remain incompletely understood. In our previous study, DT was shown to induce cytotoxic and apoptotic responses in HT\u201029 colorectal cancer cells, characterized by the upregulation of pro\u2010apoptotic genes and suppression of anti\u2010apoptotic signaling. Building on these findings, the present study aimed to explore the potential molecular context underlying DT\u2010induced transcriptional alterations using computational and bioinformatic approaches. Molecular protein\u2013protein docking was performed to evaluate the possible interaction between DT and the anti\u2010apoptotic protein Bcl\u20102. In parallel, previously obtained qRT\u2010PCR gene expression data from HT\u201029 cells were reanalyzed to assess coordinated transcriptional responses associated with DT exposure. Network analysis using the STRING database indicated that DT\u2010responsive genes form a functionally connected interaction network associated with apoptosis, cellular stress responses, and cell\u2010cycle regulation. Principal component analysis and hierarchical clustering confirmed consistent differences in gene expression patterns between treated and control samples. Docking results suggested a potential interaction between DT and the BH3\u2010binding groove of Bcl\u20102, supported by hydrogen bonding and hydrophobic contacts. Pathway enrichment analyses further linked the analyzed genes to apoptosis\u2010related pathways, including FoxO and p53 signaling. Collectively, these findings provide an integrated framework suggesting that DT\u2010induced cytotoxicity in HT\u201029 cells may involve coordinated transcriptional responses and potential structural interaction with Bcl\u20102, highlighting regulatory networks associated with apoptosis, cellular stress, and cell\u2010cycle control.<\/jats:p>","DOI":"10.1002\/jbt.70936","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T06:59:17Z","timestamp":1780383557000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Molecular Docking and Gene Expression Analyses Suggest a Potential Interaction Between Diphtheria Toxin and Bcl\u20102 in HT\u201029 Colorectal Cancer Cells"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8120-5849","authenticated-orcid":false,"given":"Elif","family":"Ercan","sequence":"first","affiliation":[{"name":"Department of 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