{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T07:25:56Z","timestamp":1786951556710,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Nasopharyngeal cancer (NPC) is a malignant tumor that originates from mucosal epithelial cells of the nasopharynx. As an emerging cancer\u2010associated microRNA, miR\u2010138\u20105p has been shown to modulate various malignant cell malignancies. However, its precise biological function and regulatory mechanism in NPC remain to be further elucidated. Human NPC cell lines (HK\u20101 and C666\u20101) were used for in vitro study, and nude mouse xenograft models were used for in vivo investigations. A bioinformatic method was utilized for the prediction of RNA expression patterns and molecular interactions. RT\u2010qPCR was performed for the detection of miR\u2010138\u20105p and CENPN expression. Luciferase activity detection and RNA pulldown experiments were used for verification of gene interaction. Flow cytometry and colony formation assays were conducted to evaluate cell cycle distribution, proliferative capacity, and apoptosis rate. Furthermore, extracellular acidification rate, lactate generation, and glucose uptake were assessed to explore the regulatory effects of miR\u2010138\u20105p and CENPN on glycolysis in NPC cells. Protein levels of CENPN and key molecules associated with cell cycle, apoptosis, glycolysis, and the PI3K signaling pathway were quantified by western blot analysis. CENPN expression is analyzed to be upregulated in head and neck cancer samples, and its high expression is associated with unfavorable clinical prognosis. In NPC cells, miR\u2010138\u20105p was markedly downregulated and was verified to directly target the 3\u2032 untranslated region of CENPN, thereby negatively regulating its expression. Functionally, overexpressing miR\u2010138\u20105p obstructed malignant phenotype, glycolysis, and tumor growth of NPC cells, where these inhibitory effects were significantly counteracted by enforced CENPN expression. Moreover, miR\u2010138\u20105p overexpression inactivated the PI3K\/Akt\/mTOR signaling by reducing CENPN expression in NPC cells. In conlusion, miR\u2010138\u20105p inhibits cell growth, glycolysis, and tumorigenesis in NPC by directly targeting CENPN, thereby inhibiting the PI3K\/Akt\/mTOR signaling pathway. These findings highlight the potential utility of miR\u2010138\u20105p as both a diagnostic indicator and a therapeutic intervention target for NPC management.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbt.70943","type":"journal-article","created":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T06:16:54Z","timestamp":1785046614000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["MiR\u2010138\u20105p Suppresses Glucose Metabolism to Inhibit the Progression of Nasopharyngeal Carcinoma by Targeting the CENPN\/PI3K\/Akt\/mTOR Signaling"],"prefix":"10.1002","volume":"40","author":[{"given":"Hongxia","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Maternal and Child Health Hospital of Hubei Province Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4904-1299","authenticated-orcid":false,"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Hubei Provincial Hospital of Traditional Chinese Medicine Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2023.22465"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)30956-0"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-024-10176-9"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1259\/bjr.20199004"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2020.112758"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2023.5545"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12218"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/146580"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.21037\/cco.2016.03.09"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.896047"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-19-0334"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1781368"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00007.2022"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.24712"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2022.154005"},{"issue":"1","key":"e_1_2_12_17_1","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1186\/s13046-021-02191-3","article-title":"The IRF2\/CENP\u2010N\/AKT Signaling Axis Promotes Proliferation, Cell Cycling and Apoptosis Resistance in Nasopharyngeal Carcinoma Cells by Increasing Aerobic Glycolysis","volume":"40","author":"Qi C. L.","year":"2021","journal-title":"Journal of Experimental & Clinical Cancer Research: CR"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2023.2258052"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-04654-x"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S282790"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.1078504"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-024-02957-5"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.102682"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2017.104"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23094723"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apcsb.2022.11.011"},{"issue":"4","key":"e_1_2_12_27_1","first-page":"1197","article-title":"Cell Proliferation and Cancer","volume":"13","author":"L\u00f3pez\u2010S\u00e1ez J. F.","year":"1998","journal-title":"Histology and Histopathology"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.2174\/0113894501279979240101051345"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17798"},{"issue":"6","key":"e_1_2_12_30_1","first-page":"5272","article-title":"Downregulation of miR\u2011138\u20115p Promotes Non\u2011Small Cell Lung Cancer Progression by Regulating CDK8","volume":"20","author":"Xing S.","year":"2019","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-12-152"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13187-013-0486-9"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24032606"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03866-7"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-021-01859-6"},{"issue":"24","key":"e_1_2_12_36_1","first-page":"5624","article-title":"Mir\u2010138\u20105p Acts as a Tumor Suppressor by Targeting Pyruvate Dehydrogenase Kinase 1 in Human Retinoblastoma","volume":"21","author":"Wang Z.","year":"2017","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.881022"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2017.368"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110600"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.79583"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17413"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13258-021-01215-8"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-2084-3112"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2021.12671"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70943","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.70943","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70943","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T07:02:19Z","timestamp":1786950139000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.70943"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,25]]},"references-count":43,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jbt.70943"],"URL":"https:\/\/doi.org\/10.1002\/jbt.70943","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,25]]},"assertion":[{"value":"2025-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70943"}}