{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:06:48Z","timestamp":1787166408081,"version":"build-2736575974"},"reference-count":80,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A convenient synthetic route was developed for the preparation of structurally diverse quinazolinone\u2013thiazole hybrids using a common thiosemicarbazide intermediate (\n                    <jats:bold>2<\/jats:bold>\n                    ), which underwent cyclization with different electrophiles to afford three series of derivatives (\n                    <jats:bold>4a\u2013e\/6, 8a\u2013c,<\/jats:bold>\n                    and\n                    <jats:bold>11a,b\/13<\/jats:bold>\n                    ). The structures of all synthesized compounds were confirmed by IR, NMR, MS, and elemental (CHN) analyses. The cytotoxic activity of the obtained compounds was evaluated against HCT\u2010116 human colon cancer cells using the MTT assay after 24\u2009h of incubation. Compounds\n                    <jats:bold>4b<\/jats:bold>\n                    (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 3.82\u2009\u00b5M),\n                    <jats:bold>4a<\/jats:bold>\n                    (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 4.07\u2009\u00b5M), and\n                    <jats:bold>11b<\/jats:bold>\n                    (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 4.09\u2009\u00b5M) were the most active derivatives, showing activity comparable to doxorubicin (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 4.17\u2009\u00b5M), while compound\n                    <jats:bold>6<\/jats:bold>\n                    also showed good activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 5.92\u2009\u00b5M). Preliminary structure\u2013activity relationship analysis indicated that electron\u2010donating aryl substituents such as methyl and methoxy groups enhanced the activity in the 4\u2010series, whereas electron\u2010withdrawing substituents such as chloro, bromo, and nitro reduced it; in the 11\u2010series, the ethyl ester derivative\n                    <jats:bold>11b<\/jats:bold>\n                    was more potent than the corresponding acetyl derivative\n                    <jats:bold>11a<\/jats:bold>\n                    . Cytotoxicity against the non\u2010tumor LLC\u2010MK2 cell line showed that compounds\n                    <jats:bold>4a, 4b, 6,<\/jats:bold>\n                    and\n                    <jats:bold>11b<\/jats:bold>\n                    exhibited IC\n                    <jats:sub>50<\/jats:sub>\n                    values\u2009&gt;\u2009100\u2009\u00b5M, indicating a favorable preliminary selectivity toward the cancer cell line. Molecular docking against human uridine 5\u2032\u2010monophosphate synthase (UMPS, PDB: 7OUZ) supported the observed activity trends, and in silico ADMET prediction suggested acceptable drug\u2010like and pharmacokinetic properties. Overall, compounds\n                    <jats:bold>4a, 4b,<\/jats:bold>\n                    and\n                    <jats:bold>11b<\/jats:bold>\n                    emerged as promising anticancer candidates for further mechanistic and selectivity studies.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70944","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T05:55:50Z","timestamp":1780466150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Design, Synthesis, and Biological Evaluation of Novel Quinazoline\u2013Thiazole Hybrids As Potential Anticancer Agents"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7739-2837","authenticated-orcid":false,"given":"Sobhi M.","family":"Gomha","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science Islamic University of Madinah  Madinah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sami A.","family":"Al\u2010Hussain","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Imam Mohammed Ibn Saud Islamic University (IMSIU) Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basant","family":"Farag","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahlah A.","family":"Alhadhrami","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Science Taibah University Madinah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5643-9202","authenticated-orcid":false,"given":"Magdi E. 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