{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T06:47:37Z","timestamp":1782110857196,"version":"3.54.5"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Oral squamous cell carcinoma (OSCC) is the most prevalent and drug\u2010resistant cancer. We studied the chemopreventive effects of Hesperidin Methyl Chalcone (HMC) on DMBA\u2010induced hamster buccal pouch carcinogenesis (HBPCs). Male golden Syrian hamsters developed oral tumors on their left buccal pouches after being treated with 0.5% DMBA topically three times a week for 10 weeks. These findings suggest that well\u2010developed squamous cell carcinomas were highly differentiated, exhibiting hyperplasia and dysplasia. Hamsters with DMBA\u2010painted buccal mucosal tissue were treated with HMC, which greatly improved the biochemical and histological changes. In addition, in silico analysis was performed to anticipate possible protein targets for this medication, including Akt, Pi3k, GSk\u20103\u03b2, \u03b2\u2010catenin, and STAT3. HMC has a high binding affinity to the above proteins. The current study reveals that HMC has strong chemopreventive properties and protects the Akt, Pi3k, GSk\u20103\u03b2, \u03b2\u2010catenin, and STAT3 proteins, which were considerably changed during DMBA\u2010induced oral carcinogenesis.<\/jats:p>","DOI":"10.1002\/jbt.70966","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T10:34:10Z","timestamp":1781778850000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hesperidin Methyl Chalcone Inhibits Hallmarks of Cancer by Targeting the AKT, PI3K\/GSK\u20103\u03b2\/\u03b2\u2010Catenin\/STAT3 Signaling Axis in Oral Squamous Cell Carcinoma Models"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6692-1871","authenticated-orcid":false,"given":"Saranya","family":"Jawaharlal","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Faculty of Science Annamalai University Annamalainagar Tamilnadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramachandhiran","family":"Duraisamy","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Faculty of Science Annamalai University Annamalainagar Tamilnadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1755-6788","authenticated-orcid":false,"given":"Vinothkumar","family":"Veerasamy","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Faculty of Science Annamalai University Annamalainagar Tamilnadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2019.104551"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11010145"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2012.06.005"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.ijmr_1821_22"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.280"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/138955708786369564"},{"issue":"2","key":"e_1_2_10_9_1","first-page":"553","article-title":"Anticancer Potential of Diphenyl Carbonate\u2010Crosslinked Cyclodextrin Nanocarrier Loaded With Allyl Methyl Sulfide in a DMBA\u2010Induced Oral Squamous Cell Carcinoma Model","volume":"28","author":"Saranya J.","year":"2025","journal-title":"Toxicology International"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytol.2013.11.010"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2024.189207"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11605-013-2168-7"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2012.10.002"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12263-012-0281-y"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037960"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450122666210118150313"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101878"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/app10196981"},{"issue":"2","key":"e_1_2_10_19_1","first-page":"199","article-title":"\u03b2\u2010Caryophyllene Modulates the Inflammatory and Apoptotic Signaling Cascades to Alter the Cellular Response During DMBA\u2010Induced Experimental Oral Carcinogenesis: A Histological and In\u2010Silico Study","volume":"28","author":"Ramachandhiran D.","year":"2021","journal-title":"Toxicology International"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352004000600003"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02148042"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(92)90122-N"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4800(68)90041-5"},{"key":"e_1_2_10_24_1","first-page":"309","volume-title":"Methods in Enzymology","author":"Ernster L.","year":"1967"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)82244-3"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1089\/adt.2019.943"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40203-017-0023-3"},{"issue":"3","key":"e_1_2_10_29_1","first-page":"103","article-title":"Deep Learning in Molecular Docking and Drug Discovery","volume":"29","author":"Wang J.","year":"2024","journal-title":"Drug Discovery Today"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5770"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200102000-00003"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23039"},{"issue":"1","key":"e_1_2_10_33_1","first-page":"A012","article-title":"Serum Raman Spectroscopy Study in DMBA\u2010Induced Oral Carcinogenesis\u2014Hamster Buccal Pouch Model","volume":"43","author":"Jadhav P. A.","year":"2022","journal-title":"Indian Journal of Medical and Paediatric Oncology"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/360438"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3892\/or_00000803"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/13813455.2017.1317815"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/WOX.0b013e3182439613"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/10520295.2018.1493221"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-015-1096-3"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9111156"},{"issue":"3","key":"e_1_2_10_41_1","first-page":"210","article-title":"Cytochrome P450\u2010Mediated Xenobiotic Metabolism: Evolutionary Adaptability and Detoxification Mechanisms","volume":"15","author":"Shang X.","year":"2024","journal-title":"Journal of Pharmaceutics"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.15430\/JCP.2016.21.1.1"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.109.031351"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-009-9162-8"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-010-0522-6"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8050137"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70966","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.70966","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70966","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T06:04:05Z","timestamp":1782108245000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.70966"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":45,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/jbt.70966"],"URL":"https:\/\/doi.org\/10.1002\/jbt.70966","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2025-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70966"}}