{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T06:47:33Z","timestamp":1782110853878,"version":"3.54.5"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004214","name":"Pamukkale \u00dcniversitesi","doi-asserted-by":"publisher","award":["2024SABE016"],"award-info":[{"award-number":["2024SABE016"]}],"id":[{"id":"10.13039\/501100004214","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mancozeb (MNZ), a widely used dithiocarbamate fungicide, has been associated with oxidative stress\u2013mediated organ toxicity in nontarget species. The present study investigated the potential protective effects of N\u2010acetylcysteine (NAC) against MNZ\u2010induced hepatotoxicity and nephrotoxicity in rats. Thirty\u2010two adult male Wistar rats were randomly assigned to four groups: Control, NAC (200\u2009mg\/kg, i.p.), MNZ (500\u2009mg\/kg, p.o.), and MNZ\u2009+\u2009NAC for 28 days. Serum biochemical parameters, tissue oxidative status, inflammatory cytokine levels, apoptosis\u2010related gene expression, and histopathological alterations were evaluated in liver and kidney tissues. MNZ administration significantly elevated serum ALT, AST, GGT, bilirubin, creatinine, and urea levels compared with controls (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). In both liver and kidney tissues, MNZ caused a significant increase in the oxidative stress index (OSI) by elevating total oxidant status (TOS) and reducing total antioxidant status (TAS) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Pro\u2010inflammatory cytokines (IL\u20101\u03b2, IL\u20106) and the mRNA expression of TNF\u2010\u03b1, iNOS, COX\u20102, Bax, and caspase\u20103 were significantly upregulated, whereas Bcl\u20102 expression and the Bcl\u20102\/Bax ratio were reduced. Histopathological evaluation confirmed substantial structural deterioration in both organs. NAC co\u2010administration significantly attenuated biochemical abnormalities, restored redox balance, modulated inflammatory and apoptotic gene expression, and markedly improved tissue architecture. These findings indicate that MNZ induces hepatorenal injury through oxidative stress\u2013associated inflammatory and apoptotic processes, and that NAC confers significant protective effects by restoring cellular redox homeostasis and limiting downstream tissue damage.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70967","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T10:19:00Z","timestamp":1781518740000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["N\u2010Acetylcysteine Attenuates Mancozeb\u2010Induced Hepatorenal Injury by Modulating Oxidative Stress, Inflammatory Signaling, and Apoptotic Pathways in Rats"],"prefix":"10.1002","volume":"40","author":[{"given":"Ali","family":"\u00c7ak\u0131rl\u0131","sequence":"first","affiliation":[{"name":"Department of Medical Pharmacology, Faculty of Medicine Pamukkale University Denizli T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3188-8738","authenticated-orcid":false,"given":"Metin Deniz","family":"Karako\u00e7","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology, Faculty of Medicine Pamukkale University Denizli T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zlem","family":"\u00d6zmen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, Faculty of Veterinary Medicine Burdur Mehmet Akif Ersoy University Burdur T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00fcnevver Nazl\u0131can","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology, Faculty of Medicine Pamukkale University Denizli T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osman","family":"\u00c7ift\u00e7i","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology, Faculty of Medicine Pamukkale University Denizli T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c02187"},{"issue":"11","key":"e_1_2_10_3_1","first-page":"4113","article-title":"Systemic Effects of the Pesticide Mancozeb: A Literature Review","volume":"25","author":"Dall'Agnol J. 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