{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:13:50Z","timestamp":1790079230643,"version":"4.0.1"},"reference-count":64,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study investigates the antihypertensive, antioxidant, and cardioprotective effects of Aloin following Langendorff rat isolated heart technique, the main bioactive compound of\n                    <jats:italic>Aloe vera<\/jats:italic>\n                    L., using both experimental (The myocardial ischemia\u2010reperfusion (IR) model, the determination of endogenous myocardial antioxidant enzyme activities, and histopathological examination of myocardial tissue) and computational approaches. To contextualize its potential, Aloin's activity was compared with Verapamil, a standard cardiovascular drug that, despite its efficacy, is limited by side effects and contraindications, particularly in patients with heart failure or hypersensitivity. In vivo experiments demonstrated that Aloin exerts significant cardioprotective and antihypertensive effects in a dose\u2010dependent manner, in some cases matching or surpassing the efficacy of Verapamil. Aloin improved cardiac performance, as shown by increased left ventricular developed pressure (LVDP) and decreased left ventricular end\u2010diastolic pressure (LVEDP) and coronary perfusion pressure (CPP). It also enhanced antioxidant defenses by elevating superoxide dismutase (SOD) and catalase (CAT) activities, while reducing oxidative stress, evidenced by lower malondialdehyde (MDA) levels. Complementary POM analyses confirmed Aloin's non\u2010toxic profile and identified pharmacophoric features underlying its bioactivity. Collectively, these findings highlight Aloin's strong potential as a natural therapeutic agent for hypertension and cardiovascular protection, warranting further clinical evaluation.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70973","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T07:09:31Z","timestamp":1782198571000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cardioprotective Effect of Aloin Against Ischemia\/Reperfusion Injury: Experimental and Computational POM Approaches"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6326-0991","authenticated-orcid":false,"given":"Mounime","family":"Kadi","sequence":"first","affiliation":[{"name":"Laboratory of Bioressources, Biotechnology, Ethnopharmacology and Health Faculty of Sciences, Mohammed First University Oujda Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taibi Ben","family":"Hadda","sequence":"additional","affiliation":[{"name":"Euromed University of Fes (UEMF) Fez Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faisal A.","family":"Almalki","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Faculty of Pharmacy, Umm Al\u2010Qura University Makkah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nermine M.","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy, Helwan University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed I. S.","family":"Abdelhady","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy, Helwan University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reham R.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy, Helwan University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2224-0852","authenticated-orcid":false,"given":"Abhay Prakash","family":"Mishra","sequence":"additional","affiliation":[{"name":"Cosmetics and Natural Products Research (CosNat) Center, Faculty of Pharmaceutical Sciences Naresuan University Phitsanulok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6650-9158","authenticated-orcid":false,"given":"Ahmad O.","family":"Babalghith","sequence":"additional","affiliation":[{"name":"Medical Genetics Department, College of Medicine Umm Al\u2010qura University Makkah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4751-8379","authenticated-orcid":false,"given":"Ajmal R.","family":"Bhat","sequence":"additional","affiliation":[{"name":"Department of Chemistry RTM Nagpur University Nagpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8625-9504","authenticated-orcid":false,"given":"Sumeer","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Indian Institute of Integrative Medicine, Medicinal Chemistry Division Academy of Scientific and innovative Research Jammu India"},{"name":"Natural Products and Medicinal Chemistry Division CSIR\u2013Indian Institute of Integrative Medicine (IIIM)  Jammu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6384-2537","authenticated-orcid":false,"given":"Abderrahim","family":"Ziyyat","sequence":"additional","affiliation":[{"name":"Laboratory of Bioressources, Biotechnology, Ethnopharmacology and Health Faculty of Sciences, Mohammed First University Oujda Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelkhaleq","family":"Legssyer","sequence":"additional","affiliation":[{"name":"Laboratory of Bioressources, Biotechnology, Ethnopharmacology and Health Faculty of Sciences, Mohammed First University Oujda Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0463-1163","authenticated-orcid":false,"given":"Younis A.","family":"Hajam","sequence":"additional","affiliation":[{"name":"Natural Products and Medicinal Chemistry Division CSIR\u2013Indian Institute of Integrative Medicine (IIIM)  Jammu India"},{"name":"Department of Life Sciences Sant Baba Bhag Singh University Jalandhar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14020350"},{"key":"e_1_2_10_3_1","volume-title":"WHO Model Formulary 2008","author":"World Health Organization","year":"2009"},{"key":"e_1_2_10_4_1","unstructured":"Mother To Baby | Fact Sheets [Internet]. Brentwood (TN): Organization of Teratology Information Specialists (OTIS);1994. Verapamil. 2023 https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK592263\/."},{"key":"e_1_2_10_5_1","volume-title":"StatPearls [Internet]","author":"McKeever R. G.","year":"2025"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2023.1158198"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43450\u2010021\u201000175\u20100"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.22092\/jmpb.2021.355897.1395"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1087"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph15070840"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-091302-2.50032-3"},{"issue":"3","key":"e_1_2_10_12_1","first-page":"772","article-title":"Hydrogen Peroxide Changes in Ischemic and Reperfused Heart. Cytochemistry and Biochemical and X\u2010Ray Microanalysis","volume":"147","author":"Slezak J.","year":"1995","journal-title":"The American Journal of Pathology"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0076\u20106879(78)52032\u20106"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0024\u20103205(03)00432\u20106"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2022.11357"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000001191"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10071473"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2021.01.036"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.5937\/jomb0\u201028879"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jced.4c00485"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2024.107761"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2023.2226713"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2023.105465"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2022.2111360"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.958379"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2022.2090437"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jics.2022.100650"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jced.4c00485"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065\u2010015\u20100100\u20109"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2023.135191"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0273256"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2022.132683"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2020.128423"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11164\u2010014\u20101802\u20107"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044\u2010014\u20101211\u2010x"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3998\/ark.5550190.0008.g03"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2020.127990"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e02689"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2022.2111360"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctim.2016.02.002"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.06.077"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12588-019-09259-y"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044\u2010014\u20101197\u20104"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2014.08.014"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms15035115"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cdc.2019.100306"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-012-0223-7"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1039\/b009667i"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2022.132688"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2021.108246"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2022.133605"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-017-1920-z"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-012-0089-8"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.22146\/ijc.46375"},{"issue":"1","key":"e_1_2_10_56_1","first-page":"34","article-title":"Synthesis of New \u03b2\u2010lactams Bearing the Biologically Important Morpholine Ring and POM Analyses of Their Antimicrobial and Antimalarial Activities","volume":"18","author":"Jarrahpour A.","year":"2019","journal-title":"Iranian Journal of Pharmaceutical Research: IJPR"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-015-0736-y"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-013-1937-7"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-012-0010-5"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11164-016-2737-y"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.17344\/acsi.2016.2428"},{"issue":"1","key":"e_1_2_10_62_1","first-page":"281","article-title":"Synthesis and Cytotoxicity Studies of 4\u2010Alkoxychalcones as New Antitumor Agents","volume":"5","author":"Abbas A.","year":"2014","journal-title":"Journal of Materials and Environmental Science"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.12099"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.3998\/ark.5550190.0009.c05"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2021.1930161"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70973","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.70973","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70973","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:43:25Z","timestamp":1790077405000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.70973"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,23]]},"references-count":64,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/jbt.70973"],"URL":"https:\/\/doi.org\/10.1002\/jbt.70973","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,23]]},"assertion":[{"value":"2025-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70973"}}