{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:15:04Z","timestamp":1790079304890,"version":"4.0.1"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The most common cancer in women worldwide is still breast cancer. Among its subtypes, luminal A and triple\u2010negative breast cancers present significant therapeutic challenges due to intrinsic drug resistance and absence of effective targeted treatments. The pharmacological potential of heterocyclic compounds has garnered more attention in recent years, and structures like pyrazolines, hydrazones, and triazoles are important in the development of new drugs. Compounds incorporating these scaffolds have been shown in numerous studies to have strong anticancer action. In this regard, pyrazoline derivatives have become a viable field of study for focused treatment approaches. This study focused on evaluating the cytotoxic potential of the 2\u2010pyrazoline\u20105\u2010one derivative compound against luminal\u2010A (MCF\u20107) and triple\u2010negative (MDA\u2010MB\u2010231) breast cancer cell lines. The CCK\u20108 test was used to assess the impact on cell viability. Additionally, Annexin V\/PI staining was used to identify the apoptotic effects, and fluorescence microscopy was used for analysis. The wound healing experiment was used to measure the capacity of cells to migrate, and migration process was monitored following scratch formation. The colony formation assay was used to assess the potential for cell proliferation, and clonal growth capacities were compared. Bax protein levels in compound\u2010treated cancer cells were quantified by ELISA to investigate the apoptotic mechanism. IC50 values in MCF\u20107 and MDA\u2010MB\u2010231 cell lines were used to evaluate the cytotoxic effects of the 2\u2010pyrazoline\u20105\u2010one derivative. The compound exhibited strong cytotoxic activity and reduced cell viability in both cell lines in a concentration\u2010dependent manner, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 13\u2009\u00b5M in MCF\u20107 cells and 16\u2009\u00b5M in MDA\u2010MB\u2010231 cell. Furthermore, the compound effectively induced apoptosis and significantly suppressed colony formation and cell migration in both cell lines. Additionally, it showed a concentration\u2010dependent rise in Bax protein levels, suggesting a pro\u2010apoptotic action. These results confirm that the 2\u2010pyrazoline\u20105\u2010one derivative has strong antiproliferative and pro\u2010apoptotic activity through Bax activation, providing a possible pathway for the creation of targeted treatments for breast cancer.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70975","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T07:17:38Z","timestamp":1782199058000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["2\u2010Pyrazoline\u20105\u2010One Derivative Suppresses Cell Proliferation and Induces Apoptosis With Upregulation of Bax in Breast Cancer Cells"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6404-9880","authenticated-orcid":false,"given":"Sevgi Kocyigit","family":"Sevinc","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Biophysics Department K\u00fctahya Health Sciences University K\u00fctahya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u015e. G\u00fcniz","family":"K\u00fc\u00e7\u00fckg\u00fczel","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Pharmaceutical Chemistry Department Fenerbah\u00e7e University Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sevim","family":"Rollas","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Pharmaceutical Chemistry Department Marmara University Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fulya","family":"Yukcu","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Biophysics Department K\u00fctahya Health Sciences University K\u00fctahya 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