{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:32:27Z","timestamp":1792456347288,"version":"4.5.0"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Methotrexate (MTX), a widely used chemotherapeutic agent, is associated with central nervous system toxicity characterized by oxidative stress, neuroinflammation, and behavioral disturbances. The present study investigated whether curcumin mitigates MTX\u2010induced neurotoxicity by modulating redox balance and inflammatory\u2013neurotrophic signaling pathways. Twenty\u2010four male BALB\/c mice were randomly assigned to four groups: control, MTX (40\u2009mg\/kg, single intraperitoneal dose), MTX+curcumin (50\u2009mg\/kg oral pretreatment), and curcumin alone. Behavioral alterations were assessed using the open field, elevated plus maze, and tail suspension tests. Serum and hippocampal levels of tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), brain\u2010derived neurotrophic factor (BDNF), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were quantified. MTX administration induced significant anxiety\u2010 and depression\u2010like behaviors, elevated TNF\u2010\u03b1 levels, increased TOS and OSI, and reduced TAS and BDNF in both serum and hippocampal tissue (respectively,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05 and\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Curcumin pretreatment markedly attenuated these alterations, restoring redox homeostasis, suppressing TNF\u2010\u03b1 overexpression, and normalizing BDNF levels. Behavioral improvements paralleled biochemical recovery, particularly within hippocampal tissue. In conclusion, our data indicate that curcumin attenuates MTX\u2010induced neurobehavioral deficits and biochemical alterations. The protective effects of curcumin are likely mediated, at least in part, by restoring redox homeostasis and modulating TNF\u2010\u03b1\/BDNF signaling, suggesting a potential therapeutic avenue for mitigating chemotherapy\u2010induced neurotoxicity.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70980","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T12:35:31Z","timestamp":1782390931000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Curcumin Alleviates Methotrexate\u2010Induced Neurobehavioral Deficits and Modulates the Redox\u2013Inflammatory\u2013Neurotrophic Axis in Mice"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6932-2767","authenticated-orcid":false,"given":"Durmu\u015f Ali","family":"Aslanlar","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Medical Pharmacology Necmettin Erbakan University Konya Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4167-2623","authenticated-orcid":false,"given":"Mehmet","family":"Oz","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Physiology Aksaray University Aksaray Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6046-864X","authenticated-orcid":false,"given":"Mehmet Emre","family":"Ay","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Family Medicine Karamano\u011flu Mehmetbey University Karaman 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