{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:14:05Z","timestamp":1790079245646,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mastitis is a prevalent gynecological disorder in women, with\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ) serving as a primary etiological pathogen of this condition. Cryptotanshinone (CPT) has been demonstrated to exert anti\u2010oxidative stress and anti\u2010inflammatory effects, conferring therapeutic benefits for a diverse range of inflammatory diseases. However, its efficacy and underlying regulatory mechanisms in ameliorating\n                    <jats:italic>S. aureus<\/jats:italic>\n                    \u2010induced mastitis remain elusive. Therefore, the present study was designed to elucidate the protective effects and molecular regulatory mechanisms of CPT against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    \u2010induced mastitis. A murine model of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    \u2010induced mastitis was established via intramammary injection of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    , and CPT was administered to the model mice for therapeutic intervention. In vivo experiments revealed that CPT administration mitigated\n                    <jats:italic>S. aureus<\/jats:italic>\n                    \u2010induced mastitis in mice by suppressing the production of pro\u2010inflammatory cytokines and inhibiting ferroptosis. In vitro, CPT abrogated the\n                    <jats:italic>S. aureus<\/jats:italic>\n                    \u2010induced elevation of TNF\u2010\u03b1, IL\u20101\u03b2, MDA, ROS, and Fe\n                    <jats:sup>2+<\/jats:sup>\n                    levels in mouse mammary epithelial cells (MMECs); additionally, it restored the ATP and GSH levels that were reduced by\n                    <jats:italic>S. aureus<\/jats:italic>\n                    stimulation in these cells. Furthermore, CPT concentration\u2010dependently upregulated the expression of GPX4, Nrf2 and HO\u20101 in MMECs. Notably, the inhibitory effects of CPT on\n                    <jats:italic>S. aureus<\/jats:italic>\n                    \u2010induced inflammatory responses and ferroptosis were abrogated in Nrf2\u2010knockdown MMECs. In conclusion, this study demonstrates that CPT exerts a robust protective effect against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    \u2010induced mastitis by suppressing inflammatory responses and inhibiting ferroptosis through the activation of the Nrf2\/GPX4 signaling pathway.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70982","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T12:21:51Z","timestamp":1782390111000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cryptotanshinone Inhibits\n                    <i>Staphylococcus aureus<\/i>\n                    \u2010Induced Mastitis by Inhibiting Ferroptosis Through Activating Nrf2\/GPX4 Pathway"],"prefix":"10.1002","volume":"40","author":[{"given":"Zimeng","family":"Liu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Jilin Agriculture Science and 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