{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:39:03Z","timestamp":1790188743927,"version":"4.1.0"},"reference-count":59,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    With a high death rate, colorectal cancer (CRC) is the third most frequent cancer worldwide. Despite advances in medical therapeutics, effective treatments for CRC remain a significant challenge. Understanding the fundamental molecular mechanisms underlying the onset of CRC is crucial for developing innovative and effective therapeutic targets. Robinin (RB), a kaempferol derivative flavonoid glycoside isolated from\n                    <jats:italic>Astragalus<\/jats:italic>\n                    species, exhibits remarkable pharmacological properties, including antioxidative, anti\u2010inflammatory, apoptotic, and antitumor effects. Thus, the purpose of this work is to examine the anticancer effects of RB on 1, 2\u2010dimethylhydrazine (DMH)\u2010alone CRC in a rat model. Rats were assigned to six groups: Normal control (NC); CRC control (DMH, 20\u2009mg\/kg\/week, i.p.); RB treatment (DMH\u2009+\u2009RB at 5, 10, and 20\u2009mg\/kg bw), and RB\u2010alone (20\u2009mg\/kg bw) for 15 weeks. Our results revealed that reduced the volume and number of tumours, incidence of aberrant crypt foci (ACF), the number of crypts, and crypt multiplicity were elevated (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) in the DMH\u2010alone rats (100%). However, treatment with RB (5, 10, and 20\u2009mg\/kg bw) reduced (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) these pre\u2010cancerous lesions in a dose\u2010dependent manner. RB also diminished DMH\u2010alone weight loss, inflammatory cytokine levels, histopathological alterations and IHC (Bax, Caspase\u20103 and NF\u2010\u03baB), while restoring cAMP and normalising enzyme activities through the regulation of hepatic xenobiotic enzymes, inflammation, and apoptosis. Targeting important signalling pathways, such as NF\u2010\u03baB and PI3K\/Akt\/mTOR\/Ras, facilitated these results.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70983","type":"journal-article","created":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T17:09:03Z","timestamp":1782666543000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Robinin Attenuates Xenobiotic Enzymes, Inflammation, and Apoptosis on DMH\u2010Induced Colon Cancer via Modulating Ras\/PI3K\/Akt\/mTOR and NF\u2010\u03baB\/Bax\/Caspase\u20103 Signalling Pathways in Wistar Rats"],"prefix":"10.1002","volume":"40","author":[{"given":"Wang","family":"Anlei","sequence":"first","affiliation":[{"name":"Department of General Surgery, Xingtai People's Hospital Xingtai Hebei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang","family":"Kaihao","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Xingtai People's Hospital Xingtai Hebei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gong","family":"Yazhao","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Xingtai People's Hospital Xingtai Hebei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren","family":"Beibei","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Xingtai People's Hospital Xingtai Hebei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9258-1577","authenticated-orcid":false,"given":"Yang","family":"Guanglei","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Xingtai People's Hospital Xingtai Hebei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Shuqing","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Xingtai People's Hospital Xingtai Hebei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,28]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/2314808X.2023.2284445"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2022.109500"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/09731296251329683"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.45095"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43046\u2010025\u201000311\u20100"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm13010040"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.4103\/pm.pm_311_20"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.07.146"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2021.109686"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/20587392211069771"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25115806"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15082389"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241210201"},{"key":"e_1_2_12_16_1","first-page":"18","article-title":"Protective Effect of Sorafenib Against 1, 2\u2010dimethylhydrazine\u2010induced Colorectal Cancer in Balb\/C Mice","volume":"15","author":"Al Hassan M.","year":"2021","journal-title":"Oncology and Radiotherapy"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.21037\/jgo\u201022\u2010733"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00384\u2010024\u201004748\u2010y"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c01676"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119335"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.31083\/j.jin2101030"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2020.110993"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28145543"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.12.004"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2019.9996"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1043\u20102760(01)00523\u20109"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202001016RR"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.05.043"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2022.101865"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12013\u2010024\u201001456\u2010w"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9979843"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2013.11.023"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3fo30194j"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.4103\/pm.pm_270_21"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935\u2010023\u201003167\u20103"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201020063\u20100"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2025.115298"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00300234"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304\u20103835(87)90157\u20101"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12575\u2010010\u20109032\u2010x"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21772"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2025.115298"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2023.9556"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940\u20106207.CAPR\u201021\u20100531"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu13041268"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735\u2010025\u201010563\u20108"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13438"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aan4116"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2025.102666"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19051536"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201019843\u20109"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2013.10.009"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2005.06.002"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.01.064"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/760689"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxres\/tfaa004"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.1548"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13092176"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22635"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2011.03.009"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70983","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.70983","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70983","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:44:35Z","timestamp":1790077475000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.70983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,28]]},"references-count":59,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/jbt.70983"],"URL":"https:\/\/doi.org\/10.1002\/jbt.70983","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,28]]},"assertion":[{"value":"2025-12-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70983"}}