{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:15:17Z","timestamp":1790079317555,"version":"4.0.1"},"reference-count":60,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Castration\u2010resistant prostate cancer (CRPC) is a lethal stage of prostate cancer (PC). This research sought to examine the functions and underlying mechanisms of N\n                    <jats:sup>6<\/jats:sup>\n                    \u2010methyladenosine (m\n                    <jats:sup>6<\/jats:sup>\n                    A) methyltransferases in CRPC. Transcriptomic datasets from the Gene Expression Omnibus and The Cancer Genome Atlas were analyzed to identify dysregulated m\n                    <jats:sup>6<\/jats:sup>\n                    A methyltransferases. Cell Counting Kit\u20108, 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine incorporation, wound healing, Transwell, enzyme\u2010linked immunosorbent assay, reverse transcription quantitative polymerase chain reaction, western blot, m\n                    <jats:sup>6<\/jats:sup>\n                    A RNA immunoprecipitation quantitative PCR, co\u2010immunoprecipitation, and m\n                    <jats:sup>6<\/jats:sup>\n                    A quantification assays were performed to evaluate the phenotypic and molecular effects of methyltransferase\u2010like 3 (METTL3). A xenograft tumor model was constructed by subcutaneous injection of lentivirus\u2010transduced LNCaP cells into BALB\/c nude mice. Hematoxylin and eosin staining was utilized to detect histopathological alterations, and immunohistochemistry was applied to measure Ki67 expression. METTL3 and methyltransferase\u2010like 5 were upregulated, whereas methyltransferase\u2010like 4 was downregulated. METTL3 knockdown suppressed the proliferation, migration, invasion, and epithelial\u2010mesenchymal transition in LNCaP and C4\u20102 cells and suppressed xenograft tumor growth. METTL3 knockdown reduced dihydrotestosterone production and suppressed the protein expression of androgen receptor, kallikrein\u2010related peptidase 3, and FK506 binding protein 5. METTL3 enhanced the m\n                    <jats:sup>6<\/jats:sup>\n                    A methylation and transcript stability of aldo\u2010keto reductase family 1 member C3 (AKR1C3). AKR1C3 overexpression notably reversed the anti\u2010tumor effects induced by METTL3 knockdown. METTL3 acts as an epigenetic driver of CRPC by promoting AKR1C3 expression in an m\n                    <jats:sup>6<\/jats:sup>\n                    A\u2010dependent manner, highlighting the METTL3\/AKR1C3 axis as a promising treatment target.\n                  <\/jats:p>","DOI":"10.1002\/jbt.71004","type":"journal-article","created":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T17:17:19Z","timestamp":1782667039000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["METTL3 Promotes Castration\u2010Resistant Prostate Cancer Progression by Enhancing AKR1C3 Expression"],"prefix":"10.1002","volume":"40","author":[{"given":"Qihong","family":"Nie","sequence":"first","affiliation":[{"name":"Department of Oncology Ganzhou People's Hospital Ganzhou City Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Urology Ganzhou People's Hospital Ganzhou City Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Urology Ganzhou People's Hospital Ganzhou City Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Urology Ganzhou People's Hospital Ganzhou City Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongsheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology Ganzhou People's Hospital Ganzhou City Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailin","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Urology Ganzhou People's Hospital Ganzhou City Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9132-1816","authenticated-orcid":false,"given":"Chuance","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Urology Ganzhou People's Hospital Ganzhou City Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,28]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2024.11.013"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2025.01.010"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2021.12.039"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2023.100962"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-05084-1"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clgc.2024.102188"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-022-01500-4"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-024-06931-z"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-23-1954"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16071303"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.46379"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.61178"},{"issue":"3","key":"e_1_2_12_14_1","first-page":"334","article-title":"Role of USP10\/METTL3\/CXCR4 Axis in Immunotherapy of Castration\u2010Resistant Prostate Cancer","volume":"24","author":"Chen W.","year":"2025","journal-title":"Iranian Journal of Allergy, Asthma and Immunology"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-05773-5"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1378292"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24054513"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.0c00939"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neo.2024.101032"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.11.042"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw104"},{"key":"e_1_2_12_22_1","first-page":"0252","article-title":"AZGP1P2\/UBA1\/RBM15 Cascade Mediates the Fate Determinations of Prostate Cancer Stem Cells and Promotes Therapeutic Effect of Docetaxel in Castration\u2010Resistant Prostate Cancer via TPM1 m6A Modification","volume":"6","author":"Wang H.","year":"2023","journal-title":"Research; A Journal of Science and Its Applications"},{"issue":"1","key":"e_1_2_12_23_1","first-page":"176","article-title":"The ADAM9\/UBN2\/AKR1C3 Axis Promotes Resistance to Androgen\u2010Deprivation in Prostate Cancer","volume":"12","author":"Le T. 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