{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:15:32Z","timestamp":1790079332593,"version":"4.0.1"},"reference-count":52,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Di\u20102\u2010ethylhexyl phthalate (DEHP) is a common industrial chemical with endocrine\u2010disrupting properties, which is capable of causing developmental abnormalities in the male reproductive system, while the effect of DEHP on testicular cell autophagy and its specific molecular mechanism has not been fully clarified. In the present study, Leydig cells were adopted as the research cell model, and multiple experimental techniques including CCK\u20108 assay, ELISA, flow cytometry, immunofluorescence staining and Western blot were applied to explore the effects of DEHP on Leydig cell autophagy and its correlation with Arg2. The experimental results showed that DEHP exposure could inhibit the proliferation and secretory function of Leydig cells, decrease testosterone levels and trigger cell apoptosis. Meanwhile, DEHP treatment significantly elevated the autophagy level of Leydig cells, accompanied by the upregulated expression of autophagy\u2010related genes Beclin1 and LC3B. Moreover, DEHP exposure induced the upregulation of Arg2 expression in Leydig cells, and the knockdown of Arg2 could partially reverse the increased autophagy level and alleviate Leydig cell damage caused by DEHP. Further mechanistic experiments verified that Arg2 might regulate DEHP\u2010induced autophagy via the mTOR signaling pathway. This study confirms that Arg2\u2010mediated autophagy participates in the process of DEHP\u2010induced Leydig cell injury, which provides a novel theoretical basis and research perspective for further exploring the toxic mechanism of DEHP on the male reproductive system.<\/jats:p>","DOI":"10.1002\/jbt.71010","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T11:22:38Z","timestamp":1782818558000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Involvement of ARG2\u2010Mediated Autophagy in Di\u20102\u2010ethylhexyl Phthalate\u2010Induced Testicular Injury"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5953-294X","authenticated-orcid":false,"given":"Jinpu","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery Guizhou Provincial People's Hospital Guiyang Guizhou 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