{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:14:16Z","timestamp":1790079256579,"version":"4.0.1"},"reference-count":29,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Paraquat (PQ), a widely used non\u2010selective herbicide, poses serious environmental and health risks due to its strong oxidative potential. Although its toxic impacts on the liver, kidneys, and lungs are well documented, limited information exists regarding its reproductive toxicity in males. This study investigated the protective role of selenium (Se) against PQ\u2010induced testicular oxidative stress and reproductive dysfunction in male albino rats. Forty\u2010eight adult rats were randomly divided into four groups: control, Se (0.5\u2009mg\/kg), PQ (2\u2009mg\/kg), and PQ + Se (2\u2009+\u20090.5\u2009mg\/kg), administered orally for 8 weeks. Biochemical assays revealed that PQ exposure significantly (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) reduced the activities of antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GSR), while increasing reactive oxygen species (ROS) and malondialdehyde (MDA) concentrations in testicular tissues. PQ exposure also led to a significant decline in sperm count, motility, and viability, along with a reduction in testicular weight. Co\u2010administration of selenium markedly restored antioxidant enzyme levels and reduced oxidative damage, improving sperm quality and testicular integrity compared with the PQ group. Although partial stress effects persisted, selenium supplementation significantly alleviated PQ\u2010induced reproductive impairments. These findings suggest that selenium exerts strong antioxidant protection by maintaining redox balance and preserving sperm function, highlighting its potential as a therapeutic agent against herbicide\u2010induced male reproductive toxicity.\n                  <\/jats:p>","DOI":"10.1002\/jbt.71011","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T06:44:44Z","timestamp":1783320284000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Selenium Ameliorates Paraquat\u2010Induced Oxidative Stress and Reproductive Damage Through Restoration of Antioxidant Defense in Male Albino Rats"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0925-1727","authenticated-orcid":false,"given":"Muhammad Razzaq","family":"Shahid","sequence":"first","affiliation":[{"name":"Department of Zoology and Biochemistry Government College University Faisalabad  Punjab Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Shahid","family":"Mehmood","sequence":"additional","affiliation":[{"name":"Institute of Microbiology University of Agriculture Faisalabad  Punjab Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3191-2975","authenticated-orcid":false,"given":"Sajid","family":"Hussain","sequence":"additional","affiliation":[{"name":"Department of Zoology and Biochemistry Government College University Faisalabad  Punjab Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Afzal","sequence":"additional","affiliation":[{"name":"Department of Zoology and Biochemistry Government College University Faisalabad  Punjab Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayydah Suryya","family":"Sani","sequence":"additional","affiliation":[{"name":"Department of Zoology and Biochemistry Government College University Faisalabad  Punjab Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Adnan","sequence":"additional","affiliation":[{"name":"Department of Zoology and Biochemistry Government College University Faisalabad  Punjab Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Asif","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Islamia University of Bahawalpur  Punjab Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8147-8820","authenticated-orcid":false,"given":"Azhar","family":"Rafique","sequence":"additional","affiliation":[{"name":"Department of Zoology and Biochemistry Government College University Faisalabad  Punjab Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2019.05.104"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e25337"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-024-04187-2"},{"key":"e_1_2_10_5_1","first-page":"24","article-title":"Chrysin Attenuates Paraquat\u2010Mediated Testicular Oxidative Stress: Protecting Against Potential Reproductive Dysfunction in Wistar Rats","author":"Akamo A.","year":"2025","journal-title":"Journal of Natural Sciences, Engineering and Technology"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.56397\/IST.2025.02.01"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2019.05.104"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2021.153017"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21870"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2017.09.055"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.20473\/ovz.v11i2.2022.66-71"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.26538\/tjnpr\/v9i5.27"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2025.103822"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2012.10.001"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1385\/BTER:93:1-3:141"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2369"},{"issue":"1","key":"e_1_2_10_17_1","first-page":"33","article-title":"Acute Effects of Ruta graveolens L. on Sperm Parameters and DNA Integrity in Rats","volume":"13","author":"Halvaei I.","year":"2012","journal-title":"Journal of Reproduction & Infertility"},{"key":"e_1_2_10_18_1","volume-title":"Handbook of Histopathological and Histochemical Techniques: Including Meuseum Techniques","author":"Culling C. F. A.","year":"1983"},{"issue":"2","key":"e_1_2_10_19_1","first-page":"172","article-title":"A Comparison of the Oxidant\u2010Antioxidant Status of Serum and Seminal Plasma From Infertile Male Camels After Zinc, Selenium, and Vitamin E Treatment","volume":"13","author":"Ahmed A.","year":"2024","journal-title":"International Journal of Veterinary Sciences"},{"issue":"3","key":"e_1_2_10_20_1","first-page":"1045","article-title":"The Influence of Nutritional Biological Selenium Nanoparticles (SeNPs) for Controlling Clinostomum Marginatum Infection in Nile Tilapia (Oreochromis niloticus)","volume":"45","author":"Albohiri H. H.","year":"2025","journal-title":"Pakistan Veterinary Journal"},{"issue":"5","key":"e_1_2_10_21_1","first-page":"595","article-title":"Impact of Vitamin E and Selenium Prior the Ovsynch Synchronization on Reproductive Performance in Friesian Dairy Cows During Hot Season","volume":"30","author":"Al\u2010Moataz B. M.","year":"2024","journal-title":"Kafkas Universitesi Veteriner Fakultesi"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gat030"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-024-04187-2"},{"issue":"4","key":"e_1_2_10_24_1","first-page":"490","article-title":"In\u2010Vitro and in\u2010Vivo Antibacterial and Antibiofilm Efficacy of Selenium Nanoparticles Against Staphylococcus Aureus Supported With Toxicopathological and Behavioral Studies in Rats","volume":"13","author":"Ibrahim E. S.","year":"2024","journal-title":"International Journal of Veterinary Sciences"},{"issue":"3","key":"e_1_2_10_25_1","first-page":"1361","article-title":"Selenium Mitigates Paraquat\u2010Induced Hematotoxicity, Oxidative Stress, Biochemical Alterations, and Nephrotoxicity in Male Albino Rats","volume":"45","author":"Goraya M. U.","year":"2025","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2005.03.006"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.56397\/IST.2025.02.01"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.2141\/jpsa.0150133"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(04)36001-8"},{"issue":"4","key":"e_1_2_10_30_1","first-page":"1013","article-title":"Comparative Effects of Selenium Sources and Concentrations on Growth, Nutrient Absorption, and Biochemistry in Japanese Quails","volume":"44","author":"Satti S.","year":"2024","journal-title":"Pakistan Veterinary Journal"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.71011","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.71011","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.71011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:43:47Z","timestamp":1790077427000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.71011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":29,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/jbt.71011"],"URL":"https:\/\/doi.org\/10.1002\/jbt.71011","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2026-04-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71011"}}