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Human CRC cell lines SW480 and SW620 were treated with various concentrations of Picroside II (10\u2013100\u2009\u03bcM). Cell proliferation was assessed using the CCK\u20108 assay, colony formation assay, and EdU incorporation assay. Migration and invasion capacities were evaluated by wound healing and Transwell assays. The expression levels of EMT\u2010related markers (E\u2010cadherin, N\u2010cadherin, Vimentin, ZEB2) and key proteins in the Notch1 pathway (Notch1, Cleaved Notch1, RBP, HES1) were analyzed by Western blotting. Statistical analysis was performed using one\u2010way ANOVA and Student's t\u2010test. Picroside II inhibited the proliferation of SW480 and SW620 cells in a dose\u2010 and time\u2010dependent manner, reduced colony formation ability, and decreased DNA synthesis activity. Treatment with Picroside II suppressed the migratory and invasive abilities of CRC cells, accompanied by upregulation of E\u2010cadherin and downregulation of N\u2010cadherin, Vimentin, and ZEB2. Furthermore, Picroside II exposure led to a decrease in the expression of Notch1, Cleaved Notch1, RBP, and HES1 proteins in a concentration\u2010dependent manner. Picroside II suppresses CRC cell progression in vitro by inhibiting the Notch1 signaling pathway, providing a preliminary molecular basis for further in vivo investigation.<\/jats:p>","DOI":"10.1002\/jbt.71018","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T15:45:24Z","timestamp":1783525524000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Inhibition of Colorectal Cancer Cell Progression by Picroside II Through Modulation of the Notch1 Signaling Pathway"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8108-4100","authenticated-orcid":false,"given":"Rui","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Radiology, West China Second University Hospital Sichuan University Chengdu Sichuan China"},{"name":"Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University) Ministry of 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