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However, the neuroprotective effect of 3,6\u2010DHF on MPTP\u2010induced oxidative stress and neuroinflammation in a PD mouse model has not yet been investigated. In this study, we investigated whether 3,6\u2010dihydroxyphenylhydrazine (3,6\u2010DHF) is protective against MPTP\u2010induced oxidative stress and neuroinflammation and explored its potential neuroprotective mechanism. 3,6\u2010DHF was administered orally at doses of 5, 10, and 20\u2009mg\/kg\/day for 7 days. MPTP was administered at 30\u2009mg\/kg\/day via intraperitoneal injection, once daily, for 4 consecutive days, from day 4 to day 7. In vitro, 3,6\u2010DHF enhanced cell survival and suppressed inflammatory markers and NF\u2010\u03baB\/MAPK signaling pathways associated with microglial activation in LPS\u2010stimulated BV\u20102 cells. In the in vivo study, 3,6\u2010DHF reduced PD motor deficits and enhanced motor performance in the open field test, beam walking, rotarod, pole, and grip strength tests. 3,6\u2010DHF significantly reduced neuronal oxidative stress by decreasing lipid peroxidation, which in turn helped restore impaired antioxidant enzyme activity, while also enhancing the expression of Nrf2 and HO\u20101 proteins. It also increased the expression levels of the TH protein, reduced the expression of inflammatory mediators, and inhibited the activation of microglia and astrocytes induced by MPTP. These results suggest that 3,6\u2010DHF effectively modulates neuroprotective, antioxidant, and neuroinflammatory processes and improves motor functions, highlighting its potential for further exploration in PD treatment.<\/jats:p>","DOI":"10.1002\/jbt.71020","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T05:15:11Z","timestamp":1783919711000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Neuroprotective Effects of 3,6\u2010Dihydroxyflavone in LPS\u2010Stimulated BV\u20102 Microglial Cells and an MPTP\u2010Induced Mouse Model of Parkinson's Disease"],"prefix":"10.1002","volume":"40","author":[{"given":"Arulkumar","family":"Kuppamuthu","sequence":"first","affiliation":[{"name":"Department of Biotechnology School of Biosciences, Periyar University Salem Tamil Nadu 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