{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T07:25:56Z","timestamp":1786951556707,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ischemia\u2013reperfusion (I\/R) injury induces cardiomyocyte apoptosis, oxidative stress and elevated myocardial injury markers. This study aimed to investigate whether the microRNA\u2010548a\u20105p (miR\u2010548a\u20105p)\/phosphatase and tensin homolog deleted on chromosome 10 (PTEN) axis mediates the cardioprotection conferred by isoflurane (ISO). An in vitro hypoxia\/reoxygenation (H\/R) injury model was established using H9c2 cardiomyocytes, including control, H\/R and ISO pretreatment groups. We detected miR\u2010548a\u20105p and PTEN expression by RT\u2010qPCR, and evaluated cell viability, apoptosis, myocardial injury markers including creatine kinase\u2010MB (CK\u2010MB), cardiac troponin I (cTnI), lactate dehydrogenase (LDH), and reactive oxygen species (ROS) using CCK\u20108, flow cytometry, ELISA and DCFH\u2010DA staining. Dual\u2010luciferase reporter assay validated the direct binding between miR\u2010548a\u20105p and PTEN. H\/R downregulated miR\u2010548a\u20105p and upregulated PTEN, accompanied by decreased cell viability, increased apoptosis, myocardial injury and oxidative stress. 1.0% ISO pretreatment reversed these adverse alterations, restored miR\u2010548a\u20105p and suppressed PTEN expression. miR\u2010548a\u20105p inhibition attenuated ISO\u2010elicited cardioprotection, whereas PTEN silencing restored the cardioprotective capacity compromised by miR\u2010548a\u20105p inhibition. In conclusion, ISO exerts cardioprotective effects via the miR\u2010548a\u20105p\/PTEN signaling axis, offering a potential therapeutic target for I\/R injury.<\/jats:p>","DOI":"10.1002\/jbt.71041","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T18:31:20Z","timestamp":1785781880000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective Effect of Isoflurane on Hypoxia\/Reoxygenation\u2010Induced Myocardial Injury via miR\u2010548a\u20105p Regulation of PTEN"],"prefix":"10.1002","volume":"40","author":[{"given":"Ji","family":"Yao","sequence":"first","affiliation":[{"name":"Anesthesia Department The Third People's Hospital Health Care Group of Cixi Cixi Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyong","family":"Zheng","sequence":"additional","affiliation":[{"name":"Anesthesiology Department Beijing Cancer Hospital Beijing 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