{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:51:58Z","timestamp":1793296318876,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009391","name":"Kafkas \u00dcniversitesi","doi-asserted-by":"publisher","award":["2025\u2010TS\u201016"],"award-info":[{"award-number":["2025\u2010TS\u201016"]}],"id":[{"id":"10.13039\/501100009391","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Diabetes mellitus is a major endocrine disorder with rapidly increasing global prevalence and numerous systemic complications. Orientin, a flavonoid isolated from various medicinal plants, is known for its potent antioxidant and anti\u2010inflammatory properties. This study evaluated the protective effects of Orientin in a streptozotocin (STZ)\u2010induced type 1 diabetes model and in the pancreatic Beta\u2010TC6 cell line. In the in vivo phase, 42 mice were randomly divided into seven groups, and diabetes was induced by a single intraperitoneal injection of STZ (160\u2009mg\/kg). Proinflammatory (IL\u20101\u03b2, IL\u20106) and anti\u2010inflammatory (TGF\u2010\u03b2, IL\u201010) cytokines were measured by ELISA. Pancreatic tissues were evaluated histopathologically (Masson's trichrome, Hematoxylin\u2010eosin) and immunohistochemically (NF\u2010\u03baB p65), while oxidative stress parameters (SOD, CAT, GSH, and MDA) were analyzed. In addition, hepatic oxidative stress parameters were assessed, and liver tissues were examined using Periodic Acid\u2013Schiff (PAS) staining. In vitro, the protective effects of Orientin against STZ\u2010induced damage in Beta\u2010TC6 cells were investigated. Apoptotic markers (Caspase\u20103, Caspase\u20109, Bax, and Bcl\u20102) were evaluated immunohistochemically, and pyroptotic markers (IL\u20101\u03b2 and Caspase\u20101) were analyzed by ELISA. STZ\u2010induced diabetes increased NF\u2010\u03baB immunopositivity, proinflammatory cytokines, and oxidative stress in pancreatic tissue. Orientin treatment reduced proinflammatory cytokines, increased anti\u2010inflammatory cytokines, and improved oxidative stress parameters in both pancreatic and hepatic tissues. In Beta\u2010TC6 cells, Orientin upregulated Bcl\u20102 and downregulated Bax, Caspase\u20103, Caspase\u20109, Caspase\u20101, and IL\u20101\u03b2 levels. These findings suggest that Orientin protects against STZ\u2010induced pancreatic damage by modulating oxidative stress, inflammation, apoptosis, and pyroptosis, supporting its potential as a therapeutic agent for preserving beta\u2010cell integrity in diabetes.<\/jats:p>","DOI":"10.1002\/jbt.71047","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T18:50:51Z","timestamp":1784746251000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Investigation of the Efficacy of Orientin in Streptozotocin\u2010Induced Type 1 Diabetes Model and Pancreatic Beta\u2010TC6 Cells"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2642-6096","authenticated-orcid":false,"given":"Bengul Ozdemir","family":"Sarikaya","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Kafkas University Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6422-9582","authenticated-orcid":false,"given":"Seyit Ali","family":"Bingol","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Kafkas University Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.16"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1424346"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.223545"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2021.07.070"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/0115733998335480241022084655"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000524988"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-025-01690-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-025-03949-8"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2008.01.035"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.2307\/3579750"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.107189"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3898"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines9121790"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22980"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.70549"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.70323"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-025-10501-7"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/34.3.497"},{"key":"e_1_2_10_20_1","first-page":"121","volume-title":"Methods in Enzymology","author":"Aebi H.","year":"1984"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(18)32219-7"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(79)90708-7"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2025.106050"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/mi14010151"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.32607\/actanaturae.11202"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines9121790"},{"issue":"2","key":"e_1_2_10_27_1","first-page":"007","article-title":"Y\u0131ld\u0131r\u0131m Apayd\u0131n Bet\u00fcl, Ertekin Ali, Kordal\u0131 \u015eaban, Y\u0131ld\u0131r\u0131m Serkan. 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