{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:41:51Z","timestamp":1787236911452,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Occupational exposure to polycyclic aromatic hydrocarbons (PAHs) or metal fumes in the workplace may be detrimental to male fertility. The present study compared sperm concentration, total motility, strict morphology and oxidative stress and SDF levels between 81 men, controls (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200917) and workers from plastic industry workers (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200918), steel EAF process (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200915), waste incineration plants (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200916) or aluminium factories smelting in the same plant for all workplaces involved in this cross\u2010sectional research. In all treated groups, the sperm concentration, motility, normal morphology, acrosome integrity and membrane function dropped significantly compared with those in controls (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Elevated ROS, MDA and low SOD and CAT activities confirmed the presence of severe oxidoreductive imbalance, particularly in Aluminium and incineration workers. DNA breaks assayed by SCD and comet showed a clear positive exposure\u2010response relationship with the highest OTM in aluminium workers. Network pharmacologic work showed that 67 shared targets were regulated by PAH exposure and male infertility. The hub gene analysis pointed at EGFR, ESR1, RELA, PTGS2, MMP2 and MMP9 as the core hub genes while KEGG enrichment suggested that the HIF\u20101 and TNF\u2010related inflammatory pathway, along with the endocrine disruption side of stress activating signalling, was involved. Inflammatory signal activation and endocrine disturbance together seemed to end up with really severe damage to sperm DNA, and it also turned on a bunch of mechanisms through which these chemical carcinogens can exert effect. ROS and MDA were negatively correlated with motility as well as sperm concentration significantly (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), whereas antioxidant enzymes showed a positive correlation. These findings show how PAH\u2010related industrial poisoning disrupts important reproductive output through ROS\u2010mediated genetic instability and suggest the relevance of protection measures in high\u2010risk industries.\n                  <\/jats:p>","DOI":"10.1002\/jbt.71052","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T18:24:03Z","timestamp":1785781443000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Industrial Hydrocarbons Exposure Deteriorates Sperm Integrity and Reproductive Function"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0651-3435","authenticated-orcid":false,"given":"Madhan Kumar","family":"Pichandi","sequence":"first","affiliation":[{"name":"Nano and Energy Bioscience Laboratory, Department of Biotechnology Thiruvalluvar University, Serkadu Vellore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5298-1312","authenticated-orcid":false,"given":"Babujanarthanam","family":"Ranganathanan","sequence":"additional","affiliation":[{"name":"Nano and Energy Bioscience Laboratory, Department of Biotechnology Thiruvalluvar University, Serkadu Vellore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2376-8082","authenticated-orcid":false,"given":"Doraiah","family":"Abilash","sequence":"additional","affiliation":[{"name":"School of Health Sciences The Apollo University Chittoor Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0277-9536(95)80004-4"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/2278-960X.161042"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics13030151"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10934529.2018.1528035"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpe.2015.03.011"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13101181"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/annhyg\/men011"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.63332\/joph.v5i4.1116"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.5696\/2156-9614-9.21.190309"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2019.125498"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-019-1309-6"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/life11121368"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxrep.2024.101714"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.70937"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12291-025-01364-7"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520299509108204"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.29063\/ajrh2025\/v29i10.3"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00339-025-08504-z"},{"key":"e_1_2_12_20_1","first-page":"121","volume-title":"Methods in Enzymology","author":"Aebi H.","year":"1984"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.48165\/ijar.2024.45.01.12"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-819092-0.00037-6"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59745-097-9:31"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43046-024-00246-y"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKAA970"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-025-04737-4"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42544"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-71815-6_8"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.5713\/ab.21.0072"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1515\/reveh-2016-0045"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.11.001"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00763"},{"key":"e_1_2_12_33_1","volume-title":"Endogenous Antioxidants: A Review of Their Role in Oxidative Stress","author":"Aguilar T. 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