{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T07:25:55Z","timestamp":1786951555259,"version":"build-2736575974"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009096","name":"Firat \u00dcniversitesi","doi-asserted-by":"publisher","award":["TF.11.78"],"award-info":[{"award-number":["TF.11.78"]}],"id":[{"id":"10.13039\/100009096","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Trichloroethylene (TCE) is a widely used industrial solvent and a common environmental pollutant. Prolonged or high\u2010dose exposure to TCE has been associated with oxidative stress, hepatotoxicity, and carcinogenesis. Accordingly, identifying compounds capable of preventing or attenuating TCE\u2010induced hepatotoxicity has become increasingly important. Thymoquinone (TQ), a bioactive compound derived from\n                    <jats:italic>Nigella sativa<\/jats:italic>\n                    , exhibits potent antioxidant, anti\u2010inflammatory, and hepatoprotective properties. This study aimed to investigate the protective effects of TQ against TCE\u2010induced liver injury. Twenty\u2010five male Wistar\u2010Albino rats were randomly assigned to five groups: Control, TCE, TCE\u2009+\u2009TQ, TQ, and DMSO. Liver tissues were harvested, processed for histopathological evaluation, and analyzed for Heat Shock Protein 70 (HSP70) immunoreactivity. TCE exposure resulted in significant histopathological alterations, including vascular congestion, necrosis, inflammation, and sinusoidal dilatation. Moreover, TCE exposure caused a marked increase in HSP70 immunoreactivity (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) compared with the control group. In contrast, TQ treatment alleviated these histopathological alterations and significantly reduced HSP70 immunoreactivity compared with the TCE group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), indicating attenuation of hepatic cellular injury. These findings suggest that TQ may exert hepatoprotective effects against TCE\u2010induced liver injury and support further investigation of its potential role in mitigating environmentally induced hepatotoxicity.\n                  <\/jats:p>","DOI":"10.1002\/jbt.71053","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T18:25:14Z","timestamp":1785781514000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective Role of Thymoquinone Against TCE\u2010Induced Liver Damage Through HSP70 Modulation"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2496-7673","authenticated-orcid":false,"given":"Arzu","family":"G\u00fcne\u015f","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology Istanbul Medipol University, International School of Medicine Istanbul T\u00fcrkiye"},{"name":"Health Science and Technologies Research Institute (SABITA) Istanbul Medipol University Istanbul T\u00fcrkiye"},{"name":"F\u0131rat University, School of Medicine, Department of Histology and Embryology Elaz\u0131\u011f T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neriman","family":"\u00c7olako\u011flu","sequence":"additional","affiliation":[{"name":"F\u0131rat University, School of Medicine, Department of Histology and Embryology Elaz\u0131\u011f T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13273-020-00075-9"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconhyd.2026.105034"},{"key":"e_1_2_10_4_1","unstructured":"Environmental Protection Agency U. Unreasonable Risk Determination under Section 6 of the Toxic Substances Control Act (TSCA) for Trichloroethylene (TCE) December 2022 accessed February 28 2026 https:\/\/www.epa.gov\/system\/files\/documents\/2023-01\/TCE_Final%20Revised%20RD_12-21-22-FINAL-v2.pdf."},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.77215"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3233\/JPD-225047"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.161"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jhbp.549"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu13061784"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19041085"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2009.04.002"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.70110"},{"key":"e_1_2_10_13_1","first-page":"51","article-title":"Teknikleri","volume":"2","author":"Demir R.","year":"2001","journal-title":"Palme Yay\u0131nc\u0131l\u0131k"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djt107"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v18.i22.2756"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3632"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.174029"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.5505\/kjms.2024.24747"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13131"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-020-10955-1"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/10520295.2018.1453549"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.34188\/bjaerv7n4-135"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-026-60695-4"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0133-3"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.71053","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.71053","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.71053","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T07:01:50Z","timestamp":1786950110000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.71053"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":23,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jbt.71053"],"URL":"https:\/\/doi.org\/10.1002\/jbt.71053","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71053"}}