{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T09:08:22Z","timestamp":1789549702609,"version":"build-2803163510"},"reference-count":67,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Mammary tumor is one of the most common neoplasms in females and remains a major therapeutic challenge due to the high cost and adverse effects of conventional chemotherapy. Therefore, herbal products such as curcumin, baicalein and their combination are used which have anti\u2010inflammatory effects and various other pharmacological effects. The present study evaluated the effects of curcumin and baicalein against mammary tumor induced by 7,12\u2010dimethylbenz[a]anthracene (DMBA) in Wistar rats. Thirty\u2010eight virgin female rats were divided into five groups: Group I (SHAM), Group II (DMBA @ 80\u2009mg\/kg BW orally single dose), Group III (DMBA @ 80\u2009mg\/kg BW orally\u2009+\u2009curcumin @ 100\u2009mg\/kg BW orally), Group IV (DMBA @ 80\u2009mg\/kg BW orally\u2009+\u2009baicalein @10\u2009mg\/kg BW i.p.), and Group V (DMBA @80\u2009mg\/kg + curcumin @100\u2009mg\/kg BW\u2009+\u2009baicalein @10\u2009mg\/kg BW). Curcumin and baicalein treatment demonstrated significant efficacy. Only small palpable growths were observed in treated groups and mild early neoplastic alterations were detected histologically, with the lowest incidence in the combination group. Curcumin and baicalein supplementation significantly ameliorated the DMBA induced changes in the body weight, hematological parameters (Hb, PCV, TEC, TLC, DLC), biochemical parameters (AST, ALT, ALKP, Total protein, glucose, BUN, Creatinine), oxidative stress parameters (NO, LPO, SOD, and Catalase), and pro\u2010inflammatory cytokines (IL\u2010I, IL\u20106, TNF\u2010\u03b1). Moreover, immunohistochemistry and RT\u2010PCR of epithelial\u2013mesenchymal transition markers (e\u2010cadherin, vimentin, \u03b1\u2010smooth muscle actin) confirmed the ameliorative effect of curcumin and baicalein, most pronounced in the combination group. These findings suggest that curcumin and baicalein may exert synergistic beneficial effects, supporting their potential as chemopreventive agents.<\/jats:p>","DOI":"10.1002\/jbt.71065","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:22:16Z","timestamp":1787566936000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Anti\u2010Tumorigenic Effects of Curcumin and Baicalein in 7,12\u2010Dimethylbenz[a] Anthracene\u2010Induced Mammary Gland Tumorigenesis in Wistar Rats"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8829-9996","authenticated-orcid":false,"given":"Priyanka","family":"Syal","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathology, Faculty of Veterinary Pathology, College of Veterinary Science Guru Angad Dev Veterinary and Animal Science University Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nittin Dev","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, Faculty of Veterinary Pathology, College of Veterinary Science Guru Angad Dev Veterinary and Animal Science University Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geeta","family":"Devi Leishangthem","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, Faculty of Veterinary Pathology, College of Veterinary Science Guru Angad Dev Veterinary and Animal Science University Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harmanjit Singh","family":"Banga","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, Faculty of Veterinary Pathology, College of Veterinary Science Guru Angad Dev Veterinary and Animal Science University Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ilar\/ilu017"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminoncol.2017.10.012"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/0118761429263841230926014118"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810393258"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-022-01347-8"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12672-025-02198-8"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering12010007"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2025.156570"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26144244"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24900"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.18520\/cs\/v119\/i6\/962-972"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.13800"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22371"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.05.069"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00320"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(96)04224-3"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2004.149"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1971.tb00790.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2002.5676"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03714.x"},{"key":"e_1_2_9_23_1","first-page":"273","volume-title":"Methods of Enzymatic Analysis","author":"Aebi H. 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