{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T07:25:57Z","timestamp":1786951557889,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Lipopolysaccharide (LPS)\u2010induced acute kidney injury (AKI) is associated with high morbidity and mortality. The molecular mechanisms underlying sepsis\u2010associated renal injury remain incompletely understood. Sinapic acid (SA), a bioactive phenolic compound, exhibits antioxidant, anti\u2010inflammatory, and cytoprotective properties, but its nephroprotective role in LPS\u2010induced AKI has not been clarified. We evaluated the protective effects of SA in an LPS\u2010induced AKI rat model and examined its associations with autophagy\u2010related, apoptotic, inflammatory, and oxidative stress markers. AKI was induced by a single intraperitoneal injection of LPS (5\u2009mg\/kg) following 7 days of oral SA pretreatment (40\u2009mg\/kg\/day). LPS administration caused marked renal tubular injury and significantly increased serum BUN, CREA, and UA levels. SA pretreatment significantly attenuated these alterations. Moreover, LPS increased renal BECN1 immunoreactivity and circulating SQSTM1\/p62 levels, indicating alterations in autophagy\u2010related markers, together with increased renal TNF\u2010\u03b1 and Caspase\u20103 immunoreactivity. SA pretreatment significantly attenuated the LPS\u2010induced increases in these markers. LPS also increased renal MDA levels and serum total oxidant status. SA pretreatment significantly reduced renal MDA without significantly altering SOD, GPx, or total antioxidant status, indicating attenuation of lipid peroxidation rather than a generalized enhancement of antioxidant defenses. Furthermore, SA attenuated the LPS\u2010induced reductions in serum albumin and total protein and the increase in LDH. Collectively, these findings suggest that SA pretreatment attenuates LPS\u2010induced AKI and that this protective effect is accompanied by changes in autophagy\u2010related markers, reduced renal TNF\u2010\u03b1 and Caspase\u20103 immunoreactivity, and decreased renal lipid peroxidation.<\/jats:p>","DOI":"10.1002\/jbt.71075","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T19:37:40Z","timestamp":1786563460000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sinapic Acid Attenuates LPS\u2010Induced Acute Kidney Injury in Rats: Changes in Autophagy\u2010Related, Apoptotic, Inflammatory, and Oxidative Stress Markers"],"prefix":"10.1002","volume":"40","author":[{"given":"Serdar","family":"Do\u011fan","sequence":"first","affiliation":[{"name":"Department of Biochemistry Antalya City Hospital Antalya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7616-3330","authenticated-orcid":false,"given":"Hamza Malik","family":"Okuyan","sequence":"additional","affiliation":[{"name":"Department of Physiotherapy and Rehabilitation \u2010 Faculty of Health Sciences, Biomedical Technologies Application and Research Center Sakarya University of Applied Sciences Sakarya T\u00fcrkiye"},{"name":"Department of Molecular Biology and Genetics, Faculty of Science Kilis 7 Aral\u0131k University Kilis T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7670-8668","authenticated-orcid":false,"given":"Ay\u00e7a","family":"Co\u015fkun","sequence":"additional","affiliation":[{"name":"Department of Physiotherapy and Rehabilitation \u2010 Faculty of Health Sciences, Biomedical Technologies Application and Research Center Sakarya University of Applied Sciences Sakarya 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