{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T19:34:12Z","timestamp":1792956852115,"version":"4.5.0"},"reference-count":64,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cyclophosphamide (CYP) is implicated in oxidative stress (OS)\u2010related platelet destruction during chemotherapy, leading to thrombocytopenia. Antioxidants can potentially attenuate the side effects of drugs and improve therapeutic outcomes. Melatonin is highly permeable and has radical scavenging and antioxidant enzyme\u2010stimulating activity. This study investigates the influence of melatonin on platelets during CYP\u2010induced OS under ex vivo conditions. Platelets isolated from the whole blood of male Wistar rats (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095) were categorized and treated as follows: Controls (CON) and Experimentals: (i) CYP, (ii) melatonin (MEL), and (iii) CYP+melatonin (CYP\u2009+\u2009MEL). Antioxidant defenses, OS and platelet function markers were assessed. Catalase, total antioxidant capacity (TAC), and lactate dehydrogenase decreased; ATP secretion, activation and cell viability decreased; and glucose and aggregation increased in CYP compared to CON. Catalase, TAC and glucose were maintained in MEL compared to CON. However, catalase increased, thereby decreasing lipid peroxidation and enhancing activation and aggregation in CYP\u2009+\u2009MEL compared to CYP. Melatonin mitigates CYP\u2010induced cellular damage by scavenging ROS and augmenting antioxidant defenses. Platelets possess a complex network of signaling cascades, and can serve as ex vivo models to study platelet dysfunction. They can provide insights into platelet behavior under simulated physiological and pathological conditions.\n                  <\/jats:p>","DOI":"10.1002\/jbt.71079","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T15:43:07Z","timestamp":1786981387000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modulatory Role of Melatonin in Cyclophosphamide\u2010Treated Platelets: Insights From Ex Vivo Oxidative Stress Model"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1094-5310","authenticated-orcid":false,"given":"Anusha Berikai","family":"Ananthakrishna","sequence":"first","affiliation":[{"name":"Department of Biotechnology &amp; Genetics, School of Sciences JAIN (Deemed\u2010to\u2010be University) Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7733-447X","authenticated-orcid":false,"given":"Magdaline Christina","family":"Rajanand","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Genetics, School of Sciences JAIN (Deemed\u2010to\u2010be University) Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4155-0960","authenticated-orcid":false,"given":"Vani","family":"Rajashekaraiah","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Genetics, School of Sciences JAIN (Deemed\u2010to\u2010be University) Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/645460"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.159178"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21144866"},{"issue":"2","key":"e_1_2_11_5_1","first-page":"1017","article-title":"Oxidative Stress and Platelet Dysfunction","volume":"2","author":"Eitan F.","year":"2018","journal-title":"Thrombosis & Haemostasis: Research"},{"key":"e_1_2_11_6_1","volume-title":"StatPearls [Internet]","author":"Ogino M. 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