{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T09:08:21Z","timestamp":1789549701612,"version":"build-2803163510"},"reference-count":69,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Acrylamide, a chemical used in industrial applications, has been classified as an environmental toxin owing to its widespread contamination of drinking water and groundwater resources. Vanillic acid (VA) is a phenolic compound naturally occurring in numerous dietary sources and medicinal plants. VA exhibits a wide range of pharmacological properties, including antioxidant, anti\u2010inflammatory, and neuroprotective activities. This study investigated the protective effects of VA against acrylamide\u2010induced oxidative stress in\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    at developmental, biochemical, and molecular levels.\n                    <jats:italic>D. melanogaster<\/jats:italic>\n                    were exposed to acrylamide (2\u2009mM) alone or with VA at three doses (0.1, 0.5, and 1\u2009mg\/L). Acrylamide exposure elevated reactive oxygen species and lipid peroxidation, decreased antioxidant enzyme activities (SOD, CAT) and glutathione (GSH) levels, increased DNA damage by the Comet assay, upregulated mRNA expression of stress\u2010related genes (\n                    <jats:italic>SOD<\/jats:italic>\n                    ,\n                    <jats:italic>CAT<\/jats:italic>\n                    ,\n                    <jats:italic>gclc<\/jats:italic>\n                    and\n                    <jats:italic>hsp70<\/jats:italic>\n                    ) and detoxification\u2010pathway components (\n                    <jats:italic>Keap1<\/jats:italic>\n                    and\n                    <jats:italic>CncC\/Nrf2<\/jats:italic>\n                    ), and adversely affected developmental parameters (larval toxicity, crawling, climbing, survival, food intake, body weight and length). Co\u2010administration of VA dose\u2010dependently reduced lipid peroxidation and ameliorated antioxidant enzyme activities and DNA damage. VA also modulated the impaired developmental parameters of acrylamide\u2010exposed larvae. These findings indicate that VA possesses considerable protective potential against acrylamide toxicity, with important implications for environmental and human health.\n                  <\/jats:p>","DOI":"10.1002\/jbt.71088","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:02:36Z","timestamp":1787760156000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acrylamide\u2010Induced Developmental and Molecular Toxicity in\n                    <i>Drosophila melanogaster<\/i>\n                    : The Role of Vanillic Acid in CncC\/Keap1 Modulation"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1744-1091","authenticated-orcid":false,"given":"Fahriye","family":"Zemheri\u2010Navruz","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics Faculty of Science, Bart\u0131n University Bart\u0131n T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1915-9797","authenticated-orcid":false,"given":"Sinan","family":"Ince","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Faculty of Veterinary Medicine, Afyon Kocatepe University Afyonkarahisar T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5833-1377","authenticated-orcid":false,"given":"Gamze","family":"Ataman","sequence":"additional","affiliation":[{"name":"Cappadocia Vocational School Pathology Laboratory Techniques Program, Cappadocia University Nev\u015fehir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"ANPI \u201cAcrylamide National Pollutant Inventory \u201d (2020) 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