{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T19:34:18Z","timestamp":1792956858684,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      The liver is a decisive organ that is damaged by sepsis. Medicarpin (Med) is a principal wing\u2010type final product of 5\u2010deoxyisoflavone sub\u2010branch in\n                      <jats:italic>M. truncatula<\/jats:italic>\n                      , and exerts anti\u2010tumor and anti\u2010inflammatory effects in diverse diseases. This research aimed to elucidate the Med function and the prospective mechanism in sepsis\u2010induced liver injury. After treating THLE\u20102 cells with diverse Med concentrations (0, 5, 10, 20, 40, 80, 160\u2009\u03bcM), Med toxic effect on THLE\u20102 cells was checked through a Cell Counting Kit\u20108 (CCK\u20108) experiment. Based on a sepsis\u2010induced liver injury model using lipopolysaccharide (LPS) and THLE\u20102 cells, Med function in sepsis\u2010induced liver injury was verified via CCK\u20108 assay, Western blot, immunofluorescence, and ELISA. Also, Med mechanism in sepsis\u2010induced liver injury was examined via Western blot, CCK\u20108 assay, and ELISA. Furthermore, Med role in sepsis\u2010induced liver injury in vivo model was elucidated through animal experiments, hematoxylin\u2010eosin staining, ELISA, Western blot, and immunofluorescence. Med possessed no obvious impact on THLE\u20102 cell viability when Med concentration was lower than 160\u2009\u03bcM. Med alleviated repression of LPS\u2010induced THLE\u20102 cell viability, and also reduced THLE\u20102 cell pyroptosis induced by LPS. From a mechanistic perspective, our data further illustrated that LPS induced a decrease in THLE\u20102 cell viability, and Med enhanced cell viability, while this impact was partially reversed after NLRP3 overexpression, indicating that Med partially counteracted the effects of NLRP3 overexpression on cell viability. Meanwhile, in vivo experimental data further demonstrated that Med alleviated liver injury and pyroptosis in sepsis mice, mainly through reduction of ALT and AST levels, a decrease in liver injury score of mice, a reduction in IL\u20101\u03b2 and IL\u201018 levels, as well as a decrease in NLRP3, ASC, cleaved caspase\u20101, and GSDMD\u2010N protein levels. In conclusion, Med exerted its protective effect on sepsis\u2010induced liver injury through NLRP3\/GSDMD\/caspase\u20101\u2010mediated cell pyroptosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbt.71098","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:50:16Z","timestamp":1788418216000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Medicarpin Mediates the Protective Effect of Cell Pyroptosis Against Sepsis\u2010Induced Liver Injury by NLRP3\/GSDMD\/Caspase\u20101 Axis"],"prefix":"10.1002","volume":"40","author":[{"given":"Pengfei","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Critical Care Medicine Jianhu County People's Hospital Yancheng City Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4117-3987","authenticated-orcid":false,"given":"Weifeng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine Jianhu County People's Hospital Yancheng City Jiangsu Province 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