{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T05:03:45Z","timestamp":1700283825293},"reference-count":7,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,12,13]],"date-time":"2004-12-13T00:00:00Z","timestamp":1102896000000},"content-version":"vor","delay-in-days":347,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Clinical Apheresis"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This prospective study was carried out in healthy donors and patients. The performance of the CliniMACS was evaluated with the comparison of the numbers of total nucleated cell (TNC) within and over the capacity of the normal scale column. In addition, large vs. normal scale column and manual vs. automated washing systems were also compared. A total of 44 selections were done. Eighteen normal scale selections were done with initial TNC numbers over 6 \u00d7 10<jats:sup>10<\/jats:sup> and 14 selections were performed below this number. None of the cases had CD34<jats:sup>+<\/jats:sup> cell numbers over the capacity. Flow cytometry was used to check each separation performance for purity and recovery of CD34<jats:sup>+<\/jats:sup> cells along with T\u2010 and B\u2010cell depletion level parameters. All healthy donors had significantly better mean purity and recovery of CD34<jats:sup>+<\/jats:sup> cells, and T\u2010 and B\u2010cell depletion status than that of patients with values 95 vs. 85%, <jats:italic>P<\/jats:italic>: 0.006; 77 vs. 58%, <jats:italic>P<\/jats:italic>: 0.004; 4.55 log vs. 4.06 log, <jats:italic>P<\/jats:italic>: 0.004; 3.19 log vs. 2.63 log, <jats:italic>P<\/jats:italic>: 0.01, respectively. However, the performance of the system was not dependent on using the normal or large\u2010scale column; automated or manual washing systems; and initial TNC numbers above (&gt;6 \u00d7 10<jats:sup>10<\/jats:sup>, range: 7.05\u201321.84 \u00d7 10<jats:sup>10<\/jats:sup>, mean: 12.32 \u00d7 10<jats:sup>10<\/jats:sup> ) or within (&lt;6 \u00d7 10<jats:sup>10<\/jats:sup>, range: 0.86\u20135.89 \u00d7 10<jats:sup>10<\/jats:sup>, mean: 4.15 \u00d7 10<jats:sup>10<\/jats:sup>) the column capacity. In conclusion, the performance of the CliniMACS is more efficient in healthy donors than in patients. However, the performance of the system did not change as long as the numbers of CD34<jats:sup>+<\/jats:sup> cells (range: 0.34\u20135.87 \u00d7 10<jats:sup>8<\/jats:sup>) were not exceeding the column capacity despite that more than 6 \u00d7 10<jats:sup>10<\/jats:sup> TNCs were used. J. Clin. Apheresis 19:197\u2013201, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jca.20029","type":"journal-article","created":{"date-parts":[[2004,12,14]],"date-time":"2004-12-14T07:16:23Z","timestamp":1103008583000},"page":"197-201","source":"Crossref","is-referenced-by-count":6,"title":["The excessive numbers of total nucleated cells does not affect the performance of the CliniMACS"],"prefix":"10.1002","volume":"19","author":[{"given":"Fikret","family":"Arpaci","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00fcrker","family":"\u00c7etin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"\u00d6zet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaan","family":"Oysul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aysel","family":"Pekel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selmin","family":"Ataergin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Okan","family":"Kuzhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u015eeref","family":"K\u00f6m\u00fcrc\u00fc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bekir","family":"\u00d6zt\u00fcrk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,12,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/152581600419242"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/15258160260194875"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1702136"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1089\/106161299320488"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1702077"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1701228"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.1.1997.6.5"}],"container-title":["Journal of Clinical Apheresis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjca.20029","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jca.20029","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T02:51:05Z","timestamp":1700275865000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jca.20029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":7,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1002\/jca.20029"],"URL":"https:\/\/doi.org\/10.1002\/jca.20029","archive":["Portico"],"relation":{},"ISSN":["0733-2459","1098-1101"],"issn-type":[{"value":"0733-2459","type":"print"},{"value":"1098-1101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]}}}