{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T05:23:46Z","timestamp":1766467426289},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,23]],"date-time":"2009-01-23T00:00:00Z","timestamp":1232668800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Clinical Apheresis"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Given that pre\u2010apheresis CD34<jats:sup>+<\/jats:sup> cell count (PA\u2010CD34) predicts the apheresis' yield, a minimum of 5 to 20 PA\u2010CD34\/\u03bcl is required in many institutions to initiate cell collection. The aim of this study was to clarify whether large\u2010volume\u2010apheresis (LVA) could facilitate progenitor cell transplantation in patients with low PA\u2010CD34. Apheresis was initiated in 226 patients, disregarding PA\u2010CD34, at days: +5 in G\u2010CSF, +10 in cyclophosphamide+G\u2010CSF, and +15 to +20 in other chemotherapy+G\u2010CSF mobilization, when leucocytes &gt;2.5 \u00d7 10<jats:sup>9<\/jats:sup>\/L. Four times the blood volume was processed. Patients were grouped according to their PA\u2010CD34: \u226510\/\u03bcl (group\u2010A, n = 143); &lt;10\/\u03bcl but \u22655\/\u03bcl (group\u2010B, n = 40) and &lt;5\/\u03bcl (group\u2010C, n = 43). No differences were found in diagnoses, gender, age, previous treatments or mobilization regimen between groups. Enough CD34<jats:sup>+<\/jats:sup> cells (&gt;1.9 \u00d7 10<jats:sup>6<\/jats:sup>\/kg) were obtained in 31 patients (72%) from group\u2010C, although in this group two mobilizations were needed in 20 patients (46.5%), compared to 5 (3.5%) and 1 (2.5%) in groups A and B, respectively (<jats:italic>P<\/jats:italic> &lt; 0.01). Evenly three apheresis or more were required in 28 patients (65.1%) from group\u2010C, compared to 8 (5.6%) and 6 (15.0%) in groups A and B, respectively (<jats:italic>P<\/jats:italic> &lt; 0.01). In conclusion LVA can facilitate autologous transplantation in poor\u2010mobilizer\u2010patients, low PA\u2010CD34 should not be an inflexible exclusion factor. J. Clin. Apheresis, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jca.20191","type":"journal-article","created":{"date-parts":[[2009,1,23]],"date-time":"2009-01-23T20:10:32Z","timestamp":1232741432000},"page":"12-17","source":"Crossref","is-referenced-by-count":20,"title":["Large\u2010volume\u2010apheresis facilitates autologous transplantation of hematopoietic progenitors in poor mobilizer patients"],"prefix":"10.1002","volume":"24","author":[{"given":"Maria 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