{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T07:10:45Z","timestamp":1696403445962},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,9,12]],"date-time":"2014-09-12T00:00:00Z","timestamp":1410480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fresenius Kabi"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Clinical Apheresis"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p><jats:bold>Background<\/jats:bold>: The ability to cryopreserve a portion of the cells treated during extracorporeal photopheresis (ECP) would improve therapy logistics, particularly for pediatric patients, by allowing multiple therapeutic doses to be collected from a single apheresis session. However, the effect of cryopreservation on ECP\u2010treated cells is unknown (e.g., ECP\u2010induced lymphocyte apoptosis and inhibition of proliferation). Study Design and <jats:bold>Methods<\/jats:bold>: Mononuclear cell (MNC) apheresis products collected from healthy subjects were ECP\u2010treated using offline methods. Fresh samples of ECP\u2010treated and control cells were placed immediately in culture. The remainder of the cells were frozen in cryovials (<jats:italic>n<\/jats:italic>\u2009=\u20098) or cryobags (<jats:italic>n<\/jats:italic>\u2009=\u20098) at \u221280\u00b0C. After 1 week of \u221280\u00b0C storage, ECP\u2010treated and control cells were thawed rapidly and samples were placed in culture. Lymphocyte apoptosis was assessed by phosphatidylserine exposure using Annexin V\/7\u2010AAD labeling. Lymphocyte proliferation after 3 days culture was measured using the carboxyfluorescein succinimidyl ester labeling technique. <jats:bold>Results<\/jats:bold>: On Day 0, apoptosis levels were &lt;5% in fresh ECP\u2010treated and control cells and approximately 20% on thawing of cryopreserved ECP\u2010treated and control cells. Apoptosis levels were comparable between the two cryopreserved groups immediately on thawing, indicating that ECP\u2010treated cells were no more sensitive to the cryopreservation process than control cells. During 72\u2010h culture, apoptosis levels increased to &gt;80% in fresh and cryopreserved ECP\u2010treated cells but remained near constant in both control groups. Inhibition of lymphocyte proliferation was &gt;95% in all ECP\u2010treated cells with no significant difference between fresh and cryopreserved cells (<jats:italic>P<\/jats:italic>\u2009=\u20090.12). <jats:bold>Conclusion<\/jats:bold>: Cryopreservation did not impair the apoptotic response or anti\u2010proliferative effect of ECP\u2010treated lymphocytes, thereby demonstrating early feasibility of this approach. J. Clin. Apheresis 30:154\u2013161, 2015. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jca.21354","type":"journal-article","created":{"date-parts":[[2014,9,12]],"date-time":"2014-09-12T06:38:15Z","timestamp":1410503895000},"page":"154-161","source":"Crossref","is-referenced-by-count":2,"title":["Cryopreserved ECP\u2010treated lymphocytes maintain apoptotic response and anti\u2010proliferative effect"],"prefix":"10.1002","volume":"30","author":[{"given":"Katherine","family":"Radwanski","sequence":"first","affiliation":[{"name":"Research and Development, Fresenius Kabi USA Lake Zurich Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl","family":"Heber","sequence":"additional","affiliation":[{"name":"Research and Development, Fresenius Kabi USA Lake Zurich Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyungyoon","family":"Min","sequence":"additional","affiliation":[{"name":"Research and Development, Fresenius Kabi USA Lake Zurich Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.20300"},{"key":"e_1_2_6_3_1","first-page":"19","article-title":"Extracorporeal photophereis in children with acute and chronic GVHD","volume":"25","author":"Witt V","year":"2010","journal-title":"J Clin Apheresis"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/14653249.2010.501787"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.03013.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2002.04560.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-0502(02)00101-5"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2009.10.002"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41050667.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.20231"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02289.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000018472"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"4009","DOI":"10.1182\/blood.V96.12.4009","article-title":"The expression of CD10 by apoptotic lymphocytes is preceded by a pronounced externalization of phosphatidylserine","volume":"96","author":"Bladon J","year":"2000","journal-title":"Blood"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12329711"}],"container-title":["Journal of Clinical Apheresis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjca.21354","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jca.21354","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T12:28:04Z","timestamp":1696336084000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jca.21354"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,12]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1002\/jca.21354"],"URL":"https:\/\/doi.org\/10.1002\/jca.21354","archive":["Portico"],"relation":{},"ISSN":["0733-2459","1098-1101"],"issn-type":[{"value":"0733-2459","type":"print"},{"value":"1098-1101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,12]]}}}