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In this report we describe a protocol for mini buffy coat preparation using the automated Sepax laboratory separator for \u201coff line\u201d ECP treatment in a low body weight child with graft\u2010vs\u2010host\u2010disease. According to our results this alternative method has been proven feasible and tolerable.<\/jats:p>","DOI":"10.1002\/jca.21700","type":"journal-article","created":{"date-parts":[[2019,3,4]],"date-time":"2019-03-04T10:44:07Z","timestamp":1551696247000},"page":"468-473","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["An automated mini buffy coat preparation method for use in mini extracorporeal photopheresis treatment of graft\u2010vs\u2010host\u2010disease in a low body weight pediatric patient"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0529-1719","authenticated-orcid":false,"given":"Toni","family":"Matic","sequence":"first","affiliation":[{"name":"Department of Pediatrics University Hospital Center Zagreb  Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2985-7847","authenticated-orcid":false,"given":"Ines","family":"Bojanic","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Transplantation Biology University Hospital Center Zagreb  Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanja","family":"Mazic","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Transplantation Biology University Hospital Center Zagreb  Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branka","family":"Golubic Cepulic","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Transplantation Biology University Hospital Center Zagreb  Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernest","family":"Bilic","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University Hospital Center Zagreb  Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,4]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01469.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.20231"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21633"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21534"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02289.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2004.10.020"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21455"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.2174\/157488812799859856"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04401.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2012.04.005"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12596"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14506"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-9987.2007.00421.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(96)07432-5"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2015.09.011"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jval.2015.01.009"}],"container-title":["Journal of Clinical Apheresis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjca.21700","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jca.21700","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T06:23:49Z","timestamp":1694327029000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jca.21700"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,4]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,8]]}},"alternative-id":["10.1002\/jca.21700"],"URL":"https:\/\/doi.org\/10.1002\/jca.21700","archive":["Portico"],"relation":{},"ISSN":["0733-2459","1098-1101"],"issn-type":[{"value":"0733-2459","type":"print"},{"value":"1098-1101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,4]]},"assertion":[{"value":"2017-08-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}