{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:06:16Z","timestamp":1778630776128,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T00:00:00Z","timestamp":1600300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI112433"],"award-info":[{"award-number":["AI112433"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI129703"],"award-info":[{"award-number":["AI129703"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI140888"],"award-info":[{"award-number":["AI140888"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["OD011092"],"award-info":[{"award-number":["OD011092"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["OD026561"],"award-info":[{"award-number":["OD026561"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Clinical Apheresis"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Macaques are physiologically relevant animal models of human immunology and infectious disease that have provided key insights and advanced clinical treatment in transplantation, vaccinology, and HIV\/AIDS. However, the small size of macaques is a stumbling block for studies requiring large numbers of cells, such as hematopoietic stem cells (HSCs) for transplantation, antigen\u2010specific lymphocytes for in\u2010depth immunological analysis, and latently\u2010infected CD4+ T\u2010cells for HIV cure studies. Here, we provide a detailed protocol for collection of large numbers of HSCs and T\u2010cells from cynomolgus macaques as small as 3 kg using the Terumo Spectra Optia apheresis system, yielding an average of 5.0\u2009\u00d7\u200910<jats:sup>9<\/jats:sup> total nucleated cells from mobilized animals and 1.2\u2009\u00d7\u200910<jats:sup>9<\/jats:sup> total nucleated cells from nonmobilized animals per procedure. This report provides sufficient detail to adapt this apheresis technique at other institutions, which will facilitate more efficient and detailed analysis of HSCs and their progeny blood cells.<\/jats:p>","DOI":"10.1002\/jca.21842","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T19:08:54Z","timestamp":1600369734000},"page":"67-77","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Terumo spectra optia leukapheresis of cynomolgus macaques for hematopoietic stem cell and T cell collection"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7101-5160","authenticated-orcid":false,"given":"Helen L.","family":"Wu","sequence":"first","affiliation":[{"name":"Vaccine &amp; Gene Therapy Institute Oregon Health &amp; Science University  Beaverton Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin 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C.","family":"Weber","sequence":"additional","affiliation":[{"name":"Vaccine &amp; Gene Therapy Institute Oregon Health &amp; Science University  Beaverton Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine B.","family":"Bateman","sequence":"additional","affiliation":[{"name":"Vaccine &amp; Gene Therapy Institute Oregon Health &amp; Science University  Beaverton Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas M.","family":"Maier","sequence":"additional","affiliation":[{"name":"Vaccine &amp; Gene Therapy Institute Oregon Health &amp; Science University  Beaverton Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mina","family":"Northrup","sequence":"additional","affiliation":[{"name":"Vaccine &amp; Gene Therapy Institute Oregon Health &amp; Science University  Beaverton Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfred 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