{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T01:20:42Z","timestamp":1775870442015,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017981","name":"Sysmex Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017981","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Clinical Apheresis"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Stem cell apheresis in the context of autologous stem cell transplantation requires an accurate cluster of differentiantion 34 (CD34<jats:sup>+<\/jats:sup>) count determined by flow cytometry as the current gold standard. Since flow cytometry is a personnel and time\u2010intensive diagnostic tool, automated stem cell enumeration may provide a promising alternative. Hence, this study aimed to compare automated hematopoietic progenitor enumeration carried out on a Sysmex XN\u201020 module compared with conventional flow cytometric measurements.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>One hundred forty\u2010three blood samples from 41 patients were included in this study. Correlation between the two methods was calculated over all samples, depending on leukocyte count and diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, we found a high degree of correlation (<jats:italic>r<\/jats:italic>\u2009=\u20090.884). Furthermore, correlation was not impaired by elevated leukocyte counts (&gt;10\u2009000\/\u03bcL, <jats:italic>r<\/jats:italic>\u2009=\u20090.860 vs &lt;10\u2009000\/\u03bcL, <jats:italic>r<\/jats:italic>\u2009=\u20090.849; &gt;20\u2009000\/\u03bcL, <jats:italic>r<\/jats:italic>\u2009=\u20090.843 vs &lt;20\u2009000\/\u03bcL, <jats:italic>r<\/jats:italic>\u2009=\u20090.875). However, correlation was significantly impaired in patients with multiple myeloma (multiple myeloma <jats:italic>r<\/jats:italic>\u2009=\u20090.840 vs nonmyeloma <jats:italic>r<\/jats:italic>\u2009=\u20090.934).<\/jats:p><\/jats:sec><jats:sec><jats:title>Summary<\/jats:title><jats:p>Stem cell measurement carried out on the Sysmex XN\u201020 module provides a significant correlation with flow cytometry and might be implemented in clinical practice. In clinical decision\u2010making, there was discrepancy of under 15% of cases. In multiple myeloma patients, XN\u201020 should be used with caution.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jca.22114","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T08:33:29Z","timestamp":1714984409000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of peripheral blood automated hematopoietic progenitor cell count and flow cytometric <scp>CD34<\/scp>+ cell count"],"prefix":"10.1002","volume":"39","author":[{"given":"Mischa","family":"Hagenk\u00f6tter","sequence":"first","affiliation":[{"name":"Department of Medicine, Hematology and Oncology, UK Knappschaftskrankenhaus Bochum GmbH Ruhr University of Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Mika","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology and Oncology, UK Knappschaftskrankenhaus Bochum GmbH Ruhr University of Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Swetlana","family":"Ladigan\u2010Badura","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology and Oncology, UK Knappschaftskrankenhaus Bochum GmbH Ruhr University of Bochum  Bochum Germany"},{"name":"Department of Hematology and Oncology Evangelisches Krankenhaus Hamm  Hamm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Schork","sequence":"additional","affiliation":[{"name":"Medizinisches Proteom\u2010Center Ruhr University Bochum  Bochum Germany"},{"name":"Medical Proteome Analysis, Center for Protein Diagnostics (PRODI) Ruhr\u2010University Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Eisenacher","sequence":"additional","affiliation":[{"name":"Medizinisches Proteom\u2010Center Ruhr University Bochum  Bochum Germany"},{"name":"Medical Proteome Analysis, Center for Protein Diagnostics (PRODI) Ruhr\u2010University Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Schroers","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology and Oncology, UK Knappschaftskrankenhaus Bochum GmbH Ruhr University of Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Baraniskin","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology and Oncology, UK Knappschaftskrankenhaus Bochum GmbH Ruhr University of Bochum  Bochum Germany"},{"name":"Department of Hematology and Oncology Evangelisches Krankenhaus Hamm  Hamm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,6]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM195709122571102"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-12-322214"},{"key":"e_1_2_12_4_1","unstructured":"DRST.Deutsches Register f\u00fcr Stammzelltransplantationen e. 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