{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T12:59:48Z","timestamp":1777294788068,"version":"3.51.4"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Clinical Apheresis"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Successful hematopoietic stem cell transplantation relies on the collection of a sufficient number of CD34\n                    <jats:sup>+<\/jats:sup>\n                    hematopoietic progenitor cells (HPCs). While acid citrate dextrose solution A (ACD\u2010A) is a common anticoagulant (AC) for HPC apheresis [HPC(A)], recent evidence suggests that concomitant heparin may enhance intra\u2010apheresis recruitment (IAR) of CD34\n                    <jats:sup>+<\/jats:sup>\n                    cells and improve collection efficiency. This study aimed to compare citrate\u2010only and citrate\u2013heparin protocols in routine HPC(A) procedures, with a focus on their effects on IAR and efficiency outcomes. A retrospective analysis was performed on 266 HPC(As) from 193 apheresis donors in autologous and allogeneic settings. Donor demographics, procedural parameters, and collection outcomes were compared between the ACD\u2010A\u2013only and ACD\u2010A plus heparin protocols. The study endpoints included apheresis yield (AY\/kg), collection efficiencies (CE\n                    <jats:sub>1<\/jats:sub>\n                    , CE\n                    <jats:sub>2<\/jats:sub>\n                    , and cruCE), recruitment ratio (RR), and performance ratio (PR). Statistical analyses included Mann\u2013Whitney\n                    <jats:italic>U<\/jats:italic>\n                    tests, effect sizes, and partial least squares (PLS) regression. Heparinized procedures demonstrated higher processed total blood volume ratios, lower AC volumes, and superior outcomes across CE\n                    <jats:sub>1<\/jats:sub>\n                    (83.8% vs. 72.5%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.00027), CE\n                    <jats:sub>2<\/jats:sub>\n                    (70.8% vs. 64.2%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0055), cruCE (77.0% vs. 72.4%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001), RR (2.3 vs. 1.7,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001), PR (239.3% vs. 208.2%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001), and AY\/kg (6.75 vs. 5.23\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    CD34\n                    <jats:sup>+<\/jats:sup>\n                    cells\/kg,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0025). The effect size and PLS analyses confirmed the contribution of heparin as an adjunct AC enhancing multiple apheresis outcomes. In summary, citrate\u2013heparin anticoagulation was found to be a feasible strategy that improved key collection efficiency and IAR metrics during HPC(A).\n                  <\/jats:p>","DOI":"10.1002\/jca.70118","type":"journal-article","created":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T05:06:58Z","timestamp":1774674418000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Citrate Versus Citrate\u2013Heparin Anticoagulation in\n                    <scp>HPC<\/scp>\n                    (A): Comparative Effects on Intra\u2010Apheresis Mobilization of\n                    <scp>CD34<\/scp>\n                    <sup>+<\/sup>\n                    Hematopoietic Progenitor Cells"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8169-9914","authenticated-orcid":false,"given":"Yandy Marx","family":"Castillo\u2010Aleman","sequence":"first","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7478-0482","authenticated-orcid":false,"given":"Carlos Agustin","family":"Villegas\u2010Valverde","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6209-0393","authenticated-orcid":false,"given":"Antonio Alfonso","family":"Bencomo\u2010Hernandez","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9490-9878","authenticated-orcid":false,"given":"Shinnette","family":"Lumame","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6316-9717","authenticated-orcid":false,"given":"Charisma","family":"Castelo","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3727-586X","authenticated-orcid":false,"given":"Jay Mary","family":"Rose\u2010Roque","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5631-322X","authenticated-orcid":false,"given":"Marlene","family":"Cato","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6773-8099","authenticated-orcid":false,"given":"Nameer","family":"Al\u2010Saadawi","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7410-9269","authenticated-orcid":false,"given":"Mohamed Ibrahim","family":"Abu\u2010Haleeqa","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3256-2783","authenticated-orcid":false,"given":"Aseel Suleiman","family":"Alsaid","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9477-8089","authenticated-orcid":false,"given":"Sakher Ahmad","family":"Obeidat","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7576-947X","authenticated-orcid":false,"given":"Mansi","family":"Sachdev","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5315-608X","authenticated-orcid":false,"given":"Jayakumar David","family":"Dennison","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3375-5674","authenticated-orcid":false,"given":"Inas","family":"El\u2010Najjar","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"},{"name":"United Arab Emirates University (UAEU)  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7222-727X","authenticated-orcid":false,"given":"Rene Antonio","family":"Rivero\u2010Jimenez","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"United Arab Emirates University (UAEU)  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5373-3414","authenticated-orcid":false,"given":"Yendry","family":"Ventura\u2010Carmenate","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"United Arab Emirates University (UAEU)  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6646-5722","authenticated-orcid":false,"given":"Fatema Mohammed","family":"Al\u2010Kaabi","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC)  Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital  Abu Dhabi UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.1.1997.6.309"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.1021"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000354377"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2008.10.018"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537\u20102995.2012.03744.x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.2920010306"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.1.1995.4.11"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21222"},{"issue":"2","key":"e_1_2_11_10_1","first-page":"249","article-title":"Heparin Based Anticoagulation During Peripheral Blood Stem Cell Collection May Increase the CD34+ Cell 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Apheresis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jca.70118","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jca.70118","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jca.70118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T12:00:17Z","timestamp":1777291217000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jca.70118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,27]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/jca.70118"],"URL":"https:\/\/doi.org\/10.1002\/jca.70118","archive":["Portico"],"relation":{},"ISSN":["0733-2459","1098-1101"],"issn-type":[{"value":"0733-2459","type":"print"},{"value":"1098-1101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,27]]},"assertion":[{"value":"2025-10-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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