{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T08:20:19Z","timestamp":1787905219318,"version":"build-2784847793"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Clinical Apheresis"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cold agglutinins can result in red blood cell (RBC) agglutination and lysis at low temperatures. Temperature sensitivity during stem cell mobilization, processing, and infusion poses unique procedural challenges in patients with active cold agglutinins. A 49\u2010year\u2010old man presented at an outside hospital with severe hemolysis and was found to have an anti\u2010I cold agglutinin and a component of warm autoimmune hemolytic anemia, together with a positive COVID and Rhinovirus test. He was treated with RBC transfusions, steroids, and Rituximab. Bone marrow evaluation several months later at our institution demonstrated IgA\u2010kappa multiple myeloma and a rare, atypical B\u2010cell population of unclear significance. After induction therapy, autologous peripheral blood stem cell transplantation was recommended. The primary concerns were cold\u2010induced agglutination during apheresis collection of hematopoietic progenitor cells and during graft handling. This required coordination across hematology, apheresis, and cell therapy teams. The patient received cyclophosphamide for cytoreduction, granulocyte colony\u2010stimulating factor for mobilization, and sutimlimab for complement inhibition. The collection was performed using practical temperature mitigation strategies including environmental warming and insulation of extracorporeal components. The product was processed using prewarmed equipment, and cryopreservation was performed per institutional protocol. A total of 5.61\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    CD34\n                    <jats:sup>+<\/jats:sup>\n                    cells\/kg were collected without circuit alarms or visible agglutination. Conditioning with high\u2010dose melphalan and autologous infusion was completed without complication, with successful engraftment. This case highlights the feasibility of pragmatic, multidisciplinary temperature management strategies during stem cell collection and processing in patients with cold agglutinin disease.\n                  <\/jats:p>","DOI":"10.1002\/jca.70158","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T03:20:26Z","timestamp":1784863226000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Temperature Safeguards and Peri\u2010Procedural Strategies for Stem Cell Collection in Cold Agglutinin Disease: A Case Report and Literature Review"],"prefix":"10.1002","volume":"41","author":[{"given":"S.","family":"Bellegarde","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Wisconsin Hospital and Clinics  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Sabol","sequence":"additional","affiliation":[{"name":"Infusion Center and Apheresis University of Wisconsin Hospital and Clinics  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Eunson","sequence":"additional","affiliation":[{"name":"Cellular Therapy Laboratory University of Wisconsin Hospital and Clinics  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z. J.","family":"Gahvari","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Wisconsin\u2010Madison, and University of Wisconsin Hospital and Clinics  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9906-9694","authenticated-orcid":false,"given":"C. P.","family":"Onyenekwu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705232"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12393"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2020.1855342"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.18147"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.70029"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15719"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17038"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2023.03.003"},{"key":"e_1_2_11_10_1","first-page":"17","article-title":"IgA Plasma Cell Myeloma Presenting as Cold Agglutinin\u2010Induced Haemolytic Transfusion Reaction","volume":"17","author":"Mehdipour Dalivand M.","year":"2024","journal-title":"BML Case Reports"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25084296"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000488753"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V64.5.1000.1000"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.17532"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-03-276162"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1610632"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.18395"}],"container-title":["Journal of Clinical Apheresis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jca.70158","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jca.70158","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jca.70158","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T08:00:48Z","timestamp":1787904048000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jca.70158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,23]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jca.70158"],"URL":"https:\/\/doi.org\/10.1002\/jca.70158","archive":["Portico"],"relation":{},"ISSN":["0733-2459","1098-1101"],"issn-type":[{"value":"0733-2459","type":"print"},{"value":"1098-1101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,23]]},"assertion":[{"value":"2026-04-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70158"}}