{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:17:06Z","timestamp":1788153426050,"version":"build-2803163510"},"reference-count":12,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Clinical Apheresis"],"published-print":{"date-parts":[[2026,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Neutrophils are unintentionally co\u2010collected during HPC(A) procedures using mononuclear cell collection protocols. Although non\u2010target granulocyte co\u2010collection is generally clinically acceptable, excessive neutrophil content may compromise certain translational research applications. This study aimed to develop a predictive model for neutrophil yield during routine HPC(A). A retrospective single\u2010center analysis of 377 HPC(A) procedures performed between January 2023 and April 2026 was conducted. Donor demographics, hematological parameters, procedural characteristics, and collection efficiency (CE) metrics were analyzed. Correlations between CE\n 1<\/jats:sub>\n and CE\n 2<\/jats:sub>\n were evaluated using Spearman's rank correlation coefficient. A multivariable linear regression model incorporating pre\u2010apheresis neutrophil count, processed blood volume (pBV), and neutrophil CE\n 1<\/jats:sub>\n was developed to predict neutrophil apheresis yield. Model performance was assessed using\n R<\/jats:italic>\n 2<\/jats:sup>\n , RMSE, MAE, Bland\u2013Altman analysis, and 5\u2010fold cross\u2010validation. Strong positive correlations were observed between CE\n 1<\/jats:sub>\n and CE\n 2<\/jats:sub>\n for both CD34\n +<\/jats:sup>\n cells (\n \u03c1<\/jats:italic>\n \u2009=\u20090.836,\n p<\/jats:italic>\n \u2009<\u20090.0001) and neutrophils (\n \u03c1<\/jats:italic>\n \u2009=\u20090.865,\n p<\/jats:italic>\n \u2009<\u20090.0001). In contrast, the multivariate regression model demonstrated only modest predictive performance for neutrophil yield (\n R<\/jats:italic>\n 2<\/jats:sup>\n \u2009=\u20090.198). Compared with the conventional fixed CE\n 1<\/jats:sub>\n approach, the regression\u2010based model showed lower RMSE, reduced systematic bias, and improved agreement between predicted and observed neutrophil yields on Bland\u2013Altman analysis. Neutrophil yield during HPC(A) can be estimated using a regression model that integrates pre\u2010apheresis neutrophil count, pBV, and CE\n 1<\/jats:sub>\n . Although predictive performance remains modest, regression\u2010based approaches improve estimation accuracy compared with simplified fixed CE models and may support procedural planning and translational research applications involving non\u2010target granulocyte co\u2010collection in HPC(A) products.\n <\/jats:p>","DOI":"10.1002\/jca.70174","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:25:18Z","timestamp":1788150318000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Predicting Non\u2010Target Neutrophil Collection During\n HPC<\/scp>\n (A): Implications for Research Protocols in Apheresis"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8169-9914","authenticated-orcid":false,"given":"Yandy Marx","family":"Castillo\u2010Aleman","sequence":"first","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7478-0482","authenticated-orcid":false,"given":"Carlos Agustin","family":"Villegas\u2010Valverde","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5373-3414","authenticated-orcid":false,"given":"Yendry","family":"Ventura\u2010Carmenate","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"},{"name":"United Arab Emirates University (UAEU) Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9490-9878","authenticated-orcid":false,"given":"Shinnette","family":"Lumame","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3727-586X","authenticated-orcid":false,"given":"Jay Mary","family":"Rose\u2010Roque","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5631-322X","authenticated-orcid":false,"given":"Marlene","family":"Cato","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6375-6986","authenticated-orcid":false,"given":"Hamda","family":"Ali","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9667-2604","authenticated-orcid":false,"given":"Ruaa Mousa","family":"Ali","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"},{"name":"Yas Clinic Khalifa City (YCKC) Hospital Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2814-3179","authenticated-orcid":false,"given":"Rachely","family":"Hernandez\u2010Corrales","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7874-4989","authenticated-orcid":false,"given":"Anil","family":"Sarode","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9483-9971","authenticated-orcid":false,"given":"Yaima","family":"Zu\u00f1iga\u2010Rosales","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5659-8214","authenticated-orcid":false,"given":"Vianed","family":"Marsan\u2010Suarez","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6209-0393","authenticated-orcid":false,"given":"Antonio Alfonso","family":"Bencomo\u2010Hernandez","sequence":"additional","affiliation":[{"name":"Abu Dhabi Stem Cells Center (ADSCC) Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000441507"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2008.02.005"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/ajts.ajts_134_21"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2025.102231"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.4137\/JCD.S11038"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2023.101849"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12928"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12907"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/14653249.2011.652733"},{"issue":"3","key":"e_1_2_11_11_1","first-page":"611","article-title":"Target Value Tailored (TVT) Apheresis Approach for Blood Progenitor Cell Collection After High\u2010Dose Chemotherapy and Rh\u2010G\u2010CSF","volume":"18","author":"Mitterer M.","year":"1996","journal-title":"Bone Marrow Transplantation"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16356"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2015.11.016"}],"container-title":["Journal of Clinical Apheresis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jca.70174","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jca.70174","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jca.70174","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:25:19Z","timestamp":1788150319000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jca.70174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,30]]},"references-count":12,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/jca.70174"],"URL":"https:\/\/doi.org\/10.1002\/jca.70174","archive":["Portico"],"relation":{},"ISSN":["0733-2459","1098-1101"],"issn-type":[{"value":"0733-2459","type":"print"},{"value":"1098-1101","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,30]]},"assertion":[{"value":"2026-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70174"}}